biostudies-literatureMohl BPWellcome Trust680-693https://www.ebi.ac.uk/biostudies/studies/S-EPMC5115167biostudies-literatureUnknown97(3)Hepatitis C virus (HCV) infection has been shown to induce autophagy but the mechanisms underpinning this process remain to be elucidated. Induction of autophagy requires the class III phosphatidylinositol 3-kinase, Vps34, which produces phosphatidylinositol 3-phosphate (PI3P) within the endoplasmic reticulum (ER) membrane. This recruits proteins with PI3P binding domains such as the double-FYVE-containing protein 1 (DFCP1). DFCP1 generates cup-shaped protrusions from the ER membrane, termed omegasomes, which provide a platform for the production of autophagosomes. Here we present data demonstrating that both Vps34 and DFCP1 are required for HCV genome replication, in the context of both a subgenomic replicon and virus infection, but did not affect virus entry or initial translation. UsingThe Journal of general virologyEarly events in the generation of autophagosomes are required for the formation of membrane structures involved in hepatitis C virus genome replication.PMC5115167096670099759Harris MMankouri JBartlett CMohl BPfalseEarly events in the generation of autophagosomes are required for the formation of membrane structures involved in hepatitis C virus genome replication.Hepatitis C virus (HCV) infection has been shown to induce autophagy but the mechanisms underpinning this process remain to be elucidated. Induction of autophagy requires the class III phosphatidylinositol 3-kinase, Vps34, which produces phosphatidylinositol 3-phosphate (PI3P) within the endoplasmic reticulum (ER) membrane. This recruits proteins with PI3P binding domains such as the double-FYVE-containing protein 1 (DFCP1). DFCP1 generates cup-shaped protrusions from the ER membrane, termed omegasomes, which provide a platform for the production of autophagosomes. Here we present data demonstrating that both Vps34 and DFCP1 are required for HCV genome replication, in the context of both a subgenomic replicon and virus infection, but did not affect virus entry or initial translation. Using2016-01-01T00:00:00Z2016 Mar2025-04-19T03:03:33.707Z2019-03-26T23:38:21ZS-EPMC51151672672792410.1099/jgv.0.000387