{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7"],"submitter":["Svitashev S"],"pubmed_abstract":["Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR-Cas9 has quickly become the technology of choice for genome editing due to its simplicity, efficiency and versatility. Currently, genome editing in plants primarily relies on delivering double-strand break reagents in the form of DNA vectors. Here we report biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles. Using this method of delivery, we also demonstrate DNA- and selectable marker-free gene mutagenesis in maize and recovery of plants with mutated alleles at high frequencie"],"journal":["Nature communications"],"pagination":["13274"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5116081"],"repository":["biostudies-literature"],"pubmed_title":["Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes."],"pmcid":["PMC5116081"],"pubmed_authors":["Svitashev S","Schwartz C","Lenderts B","Young JK","Mark Cigan A"],"additional_accession":[]},"is_claimable":false,"name":"Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes.","description":"Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR-Cas9 has quickly become the technology of choice for genome editing due to its simplicity, efficiency and versatility. Currently, genome editing in plants primarily relies on delivering double-strand break reagents in the form of DNA vectors. Here we report biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles. Using this method of delivery, we also demonstrate DNA- and selectable marker-free gene mutagenesis in maize and recovery of plants with mutated alleles at high frequencie","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Nov","modification":"2025-04-25T19:17:52.688Z","creation":"2019-03-27T02:29:22Z"},"accession":"S-EPMC5116081","cross_references":{"pubmed":["27848933"],"doi":["10.1038/ncomms13274"]}}