{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(10)"],"submitter":["Takahashi M"],"pubmed_abstract":["Exposure of Arabidopsis leaves to nitrogen dioxide (NO<sub>2</sub>) results in nitration of specific chloroplast proteins. To determine whether NO<sub>2</sub> itself and/or nitrite derived from NO<sub>2</sub> can nitrate proteins, Arabidopsis thylakoid membranes were isolated and treated with NO<sub>2</sub>-bubbled or potassium nitrite (KNO<sub>2</sub>) buffer, followed by protein extraction, electrophoresis, and immunoblotting using an anti-3-nitrotyrosine (NT) antibody. NO<sub>2</sub> concentrations in the NO<sub>2</sub>-bubbled buffer were calculated by numerically solving NO<sub>2</sub> dissociation kinetic equations. The two buffers were adjusted to have identical nitrite concentrations. Both treatments yielded an NT-immunopositive band that LC/MS identified as PSBO1. The difference in the band intensity between the 2 treatments was designated nitration by NO<sub>2</sub>. Both NO<sub>2</sub> and nitrite mediated nitration of proteins, and the nitration ability per unit NO<sub>2</sub> concentration was ∼100-fold greater than that of nitrite."],"journal":["Plant signaling & behavior"],"pagination":["e1237329"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5117089"],"repository":["biostudies-literature"],"pubmed_title":["Differential abilities of nitrogen dioxide and nitrite to nitrate proteins in thylakoid membranes isolated from Arabidopsis leaves."],"pmcid":["PMC5117089"],"pubmed_authors":["Shibata T","Shigeto J","Sakamoto A","Takahashi M","Morikawa H"],"additional_accession":[]},"is_claimable":false,"name":"Differential abilities of nitrogen dioxide and nitrite to nitrate proteins in thylakoid membranes isolated from Arabidopsis leaves.","description":"Exposure of Arabidopsis leaves to nitrogen dioxide (NO<sub>2</sub>) results in nitration of specific chloroplast proteins. To determine whether NO<sub>2</sub> itself and/or nitrite derived from NO<sub>2</sub> can nitrate proteins, Arabidopsis thylakoid membranes were isolated and treated with NO<sub>2</sub>-bubbled or potassium nitrite (KNO<sub>2</sub>) buffer, followed by protein extraction, electrophoresis, and immunoblotting using an anti-3-nitrotyrosine (NT) antibody. NO<sub>2</sub> concentrations in the NO<sub>2</sub>-bubbled buffer were calculated by numerically solving NO<sub>2</sub> dissociation kinetic equations. The two buffers were adjusted to have identical nitrite concentrations. Both treatments yielded an NT-immunopositive band that LC/MS identified as PSBO1. The difference in the band intensity between the 2 treatments was designated nitration by NO<sub>2</sub>. Both NO<sub>2</sub> and nitrite mediated nitration of proteins, and the nitration ability per unit NO<sub>2</sub> concentration was ∼100-fold greater than that of nitrite.","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Oct","modification":"2025-04-19T03:03:58.148Z","creation":"2019-03-27T02:29:25Z"},"accession":"S-EPMC5117089","cross_references":{"pubmed":["27661771"],"doi":["10.1080/15592324.2016.1237329"]}}