biostudies-literatureFarrell JJNCATS NIH HHSNCI NIH HHS1485-1493https://www.ebi.ac.uk/biostudies/studies/S-EPMC5119656biostudies-literatureUnknown45(10)<h4>Objectives</h4>There is a need for validated predictive markers of gemcitabine response to guide precision medicine treatment in pancreatic cancer. We previously validated human equilibrative nucleoside transporter 1 as a predictive marker of gemcitabine treatment response using Radiation Therapy Oncology Group 9704. Controversy exists about the predictive value of gemcitabine metabolism pathway biomarkers: deoxycytidine kinase (DCK), ribonucleotide reductase 1 (RRM1), RRM2, and p53R2.<h4>Methods</h4>Radiation Therapy Oncology Group 9704 prospectively randomized 538 patients after pancreatic resection to receive either 5-fluorouracil or gemcitabine. Tumor DCK, RRM1, RRM2, and p53R protein expressions were analyzed using a tissue microarray and immunohistochemistry and correlated with tPancreasPrecision Medicine and Pancreatic Cancer: A Gemcitabine Pathway Approach.PMC5119656U24 CA114734P30 CA060553U10 CA180868U10 CA180822UL1 TR001863Zheng ZGuha CSchaefer PElsaleh HMacdonald JSRegine WFBenson ABYen YMoughan JLiu XBepler GWong JLFarrell JJLai RfalsePrecision Medicine and Pancreatic Cancer: A Gemcitabine Pathway Approach.<h4>Objectives</h4>There is a need for validated predictive markers of gemcitabine response to guide precision medicine treatment in pancreatic cancer. We previously validated human equilibrative nucleoside transporter 1 as a predictive marker of gemcitabine treatment response using Radiation Therapy Oncology Group 9704. Controversy exists about the predictive value of gemcitabine metabolism pathway biomarkers: deoxycytidine kinase (DCK), ribonucleotide reductase 1 (RRM1), RRM2, and p53R2.<h4>Methods</h4>Radiation Therapy Oncology Group 9704 prospectively randomized 538 patients after pancreatic resection to receive either 5-fluorouracil or gemcitabine. Tumor DCK, RRM1, RRM2, and p53R protein expressions were analyzed using a tissue microarray and immunohistochemistry and correlated with t2016-01-01T00:00:00Z2016 Nov2025-04-27T01:21:50.252Z2019-03-27T02:29:35ZS-EPMC51196562774872110.1097/MPA.000000000000071010.1097/mpa.0000000000000710