{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chow YY"],"funding":["Malaysian Ministry of Higher Education"],"pagination":["13-21"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5121152"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["This study aimed to establish the colonization behavior and proliferation potential of three endophytes and one pathogen <i>Ganoderma boninense</i> (Gb) introduced into oil palm ramets (host model). The endophytes selected were <i>Diaporthe phaseolorum</i> (WAA02), <i>Trichoderma asperellum</i> (T2), and <i>Penicillium citrinum</i> (BTF08). Ramets were first inoculated with 100 mL of fungal cells (10<sup>6</sup> cfu mL<sup>-</sup><sup>1</sup>) via soil drenching. For the next 7 days, ramets were sampled and subjected to three different assays to detect and identify fungal colonization, and establish their proliferation potential <i>in planta</i>. Plate assay revealed the presence of endophytes in root, stem and leaf tissues within 7 days after inoculation. Polymerase Chain Reaction (PCR) d"],"journal":["Journal of advanced research"],"pubmed_title":["Understanding colonization and proliferation potential of endophytes and pathogen <i>in planta</i> via plating, polymerase chain reaction and ergosterol assay."],"pmcid":["PMC5121152"],"funding_grant_id":["ERGS/1/2013/STWN03/MUSM/02/1"],"pubmed_authors":["Ting AS","Rahman S","Chow YY"],"additional_accession":[]},"is_claimable":false,"name":"Understanding colonization and proliferation potential of endophytes and pathogen <i>in planta</i> via plating, polymerase chain reaction and ergosterol assay.","description":"This study aimed to establish the colonization behavior and proliferation potential of three endophytes and one pathogen <i>Ganoderma boninense</i> (Gb) introduced into oil palm ramets (host model). The endophytes selected were <i>Diaporthe phaseolorum</i> (WAA02), <i>Trichoderma asperellum</i> (T2), and <i>Penicillium citrinum</i> (BTF08). Ramets were first inoculated with 100 mL of fungal cells (10<sup>6</sup> cfu mL<sup>-</sup><sup>1</sup>) via soil drenching. For the next 7 days, ramets were sampled and subjected to three different assays to detect and identify fungal colonization, and establish their proliferation potential <i>in planta</i>. Plate assay revealed the presence of endophytes in root, stem and leaf tissues within 7 days after inoculation. Polymerase Chain Reaction (PCR) d","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jan","modification":"2025-04-18T12:03:30.819Z","creation":"2019-06-06T16:38:51Z"},"accession":"S-EPMC5121152","cross_references":{"pubmed":["27895938"],"doi":["10.1016/j.jare.2016.10.008"]}}