biostudies-literatureChow YYMalaysian Ministry of Higher Education13-21https://www.ebi.ac.uk/biostudies/studies/S-EPMC5121152biostudies-literatureUnknown8(1)This study aimed to establish the colonization behavior and proliferation potential of three endophytes and one pathogen <i>Ganoderma boninense</i> (Gb) introduced into oil palm ramets (host model). The endophytes selected were <i>Diaporthe phaseolorum</i> (WAA02), <i>Trichoderma asperellum</i> (T2), and <i>Penicillium citrinum</i> (BTF08). Ramets were first inoculated with 100 mL of fungal cells (10⁶ cfu mL^-¹) via soil drenching. For the next 7 days, ramets were sampled and subjected to three different assays to detect and identify fungal colonization, and establish their proliferation potential <i>in planta</i>. Plate assay revealed the presence of endophytes in root, stem and leaf tissues within 7 days after inoculation. Polymerase Chain Reaction (PCR) dJournal of advanced researchUnderstanding colonization and proliferation potential of endophytes and pathogen <i>in planta</i> via plating, polymerase chain reaction and ergosterol assay.PMC5121152ERGS/1/2013/STWN03/MUSM/02/1Ting ASRahman SChow YYfalseUnderstanding colonization and proliferation potential of endophytes and pathogen <i>in planta</i> via plating, polymerase chain reaction and ergosterol assay.This study aimed to establish the colonization behavior and proliferation potential of three endophytes and one pathogen <i>Ganoderma boninense</i> (Gb) introduced into oil palm ramets (host model). The endophytes selected were <i>Diaporthe phaseolorum</i> (WAA02), <i>Trichoderma asperellum</i> (T2), and <i>Penicillium citrinum</i> (BTF08). Ramets were first inoculated with 100 mL of fungal cells (10⁶ cfu mL^-¹) via soil drenching. For the next 7 days, ramets were sampled and subjected to three different assays to detect and identify fungal colonization, and establish their proliferation potential <i>in planta</i>. Plate assay revealed the presence of endophytes in root, stem and leaf tissues within 7 days after inoculation. Polymerase Chain Reaction (PCR) d2017-01-01T00:00:00Z2017 Jan2025-04-18T12:03:30.819Z2019-06-06T16:38:51ZS-EPMC51211522789593810.1016/j.jare.2016.10.008