{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Atanasova K"],"funding":["NIDCR NIH HHS","NIGMS NIH HHS"],"pagination":["37643"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5121656"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6"],"pubmed_abstract":["Nucleoside-diphosphate-kinases (NDKs) are leaderless, multifunctional enzymes. The mode(s) of NDK secretion is currently undefined, while extracellular translocation of bacterial NDKs is critical for avoidance of host pathogen clearance by opportunistic pathogens such as Porphyromonas gingivalis. P. gingivalis-NDK during infection inhibits extracellular-ATP (eATP)/P2X<sub>7</sub>-receptor mediated cell death in gingival epithelial cells (GECs) via eATP hydrolysis. Furthermore, depletion of pannexin-1-hemichannel (PNX1) coupled with P2X<sub>7</sub>-receptor blocks the infection-induced eATP release in GECs, and P. gingivalis-NDK impacts this pathway. Ultrastructural and confocal microscopy of P. gingivalis-co-cultured GECs or green-fluorescent-protein (GFP)-P. gingivalis-NDK transfected GEC"],"journal":["Scientific reports"],"pubmed_title":["Nucleoside-Diphosphate-Kinase of P. gingivalis is Secreted from Epithelial Cells In the Absence of a Leader Sequence Through a Pannexin-1 Interactome."],"pmcid":["PMC5121656"],"funding_grant_id":["R01 DE019783","T90 DE021990","P30 GM103331","R56 DE016593","T32 DE017551","R01 DE016593"],"pubmed_authors":["Atanasova K","Lee J","Lee K","Yilmaz O","Roberts J","Ojcius DM"],"additional_accession":[]},"is_claimable":false,"name":"Nucleoside-Diphosphate-Kinase of P. gingivalis is Secreted from Epithelial Cells In the Absence of a Leader Sequence Through a Pannexin-1 Interactome.","description":"Nucleoside-diphosphate-kinases (NDKs) are leaderless, multifunctional enzymes. The mode(s) of NDK secretion is currently undefined, while extracellular translocation of bacterial NDKs is critical for avoidance of host pathogen clearance by opportunistic pathogens such as Porphyromonas gingivalis. P. gingivalis-NDK during infection inhibits extracellular-ATP (eATP)/P2X<sub>7</sub>-receptor mediated cell death in gingival epithelial cells (GECs) via eATP hydrolysis. Furthermore, depletion of pannexin-1-hemichannel (PNX1) coupled with P2X<sub>7</sub>-receptor blocks the infection-induced eATP release in GECs, and P. gingivalis-NDK impacts this pathway. Ultrastructural and confocal microscopy of P. gingivalis-co-cultured GECs or green-fluorescent-protein (GFP)-P. gingivalis-NDK transfected GEC","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Nov","modification":"2025-04-04T19:40:05.696Z","creation":"2019-03-27T02:29:43Z"},"accession":"S-EPMC5121656","cross_references":{"pubmed":["27883084"],"doi":["10.1038/srep37643"]}}