{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2(2)"],"submitter":["Vehus T"],"pubmed_abstract":["<h4>Aim</h4>We compared four commonly used, commercially available reverse phase nanoLC columns for identification/determination of Wnt/β-catenin-related pathway proteins.<h4>Materials & methods</h4>The columns were: Chromolith<sup>®</sup> (silica monolith; Merke Millipore, MA, USA), PepMap™ (porous particles; Thermo Fisher Scientific, MA, USA), Accucore™ (solid core particles; Thermo Fisher Scientific) and PepSwift™ (organic monolith; Thermo Fisher Scientific).<h4>Results</h4>The peak capacity of the columns varied from 100 (Pepswift) to 190 (Accucore) (for 30 min gradients). All columns enabled identification/detection of GSK3β and β-catenin in the complex samples. However, even the columns with higher peak capacities could not enable detection of the somewhat less abundant proteins AXIN"],"journal":["Future science OA"],"pagination":["FSO119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5137844"],"repository":["biostudies-literature"],"pubmed_title":["Comparison of commercial nanoliquid chromatography columns for fast, targeted mass spectrometry-based proteomics."],"pmcid":["PMC5137844"],"pubmed_authors":["Krauss S","Seterdal KE","Lundanes E","Vehus T","Wilson SR"],"additional_accession":[]},"is_claimable":false,"name":"Comparison of commercial nanoliquid chromatography columns for fast, targeted mass spectrometry-based proteomics.","description":"<h4>Aim</h4>We compared four commonly used, commercially available reverse phase nanoLC columns for identification/determination of Wnt/β-catenin-related pathway proteins.<h4>Materials & methods</h4>The columns were: Chromolith<sup>®</sup> (silica monolith; Merke Millipore, MA, USA), PepMap™ (porous particles; Thermo Fisher Scientific, MA, USA), Accucore™ (solid core particles; Thermo Fisher Scientific) and PepSwift™ (organic monolith; Thermo Fisher Scientific).<h4>Results</h4>The peak capacity of the columns varied from 100 (Pepswift) to 190 (Accucore) (for 30 min gradients). All columns enabled identification/detection of GSK3β and β-catenin in the complex samples. However, even the columns with higher peak capacities could not enable detection of the somewhat less abundant proteins AXIN","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jun","modification":"2025-04-26T02:00:35.835Z","creation":"2019-03-27T02:30:49Z"},"accession":"S-EPMC5137844","cross_references":{"pubmed":["28031966"],"doi":["10.4155/fsoa-2016-0014"]}}