biostudies-literatureUnknown2(2)Vehus T<h4>Aim</h4>We compared four commonly used, commercially available reverse phase nanoLC columns for identification/determination of Wnt/β-catenin-related pathway proteins.<h4>Materials & methods</h4>The columns were: Chromolith^® (silica monolith; Merke Millipore, MA, USA), PepMap™ (porous particles; Thermo Fisher Scientific, MA, USA), Accucore™ (solid core particles; Thermo Fisher Scientific) and PepSwift™ (organic monolith; Thermo Fisher Scientific).<h4>Results</h4>The peak capacity of the columns varied from 100 (Pepswift) to 190 (Accucore) (for 30 min gradients). All columns enabled identification/detection of GSK3β and β-catenin in the complex samples. However, even the columns with higher peak capacities could not enable detection of the somewhat less abundant proteins AXINFuture science OAFSO119https://www.ebi.ac.uk/biostudies/studies/S-EPMC5137844biostudies-literatureComparison of commercial nanoliquid chromatography columns for fast, targeted mass spectrometry-based proteomics.PMC5137844Krauss SSeterdal KELundanes EVehus TWilson SRfalseComparison of commercial nanoliquid chromatography columns for fast, targeted mass spectrometry-based proteomics.<h4>Aim</h4>We compared four commonly used, commercially available reverse phase nanoLC columns for identification/determination of Wnt/β-catenin-related pathway proteins.<h4>Materials & methods</h4>The columns were: Chromolith^® (silica monolith; Merke Millipore, MA, USA), PepMap™ (porous particles; Thermo Fisher Scientific, MA, USA), Accucore™ (solid core particles; Thermo Fisher Scientific) and PepSwift™ (organic monolith; Thermo Fisher Scientific).<h4>Results</h4>The peak capacity of the columns varied from 100 (Pepswift) to 190 (Accucore) (for 30 min gradients). All columns enabled identification/detection of GSK3β and β-catenin in the complex samples. However, even the columns with higher peak capacities could not enable detection of the somewhat less abundant proteins AXIN2016-01-01T00:00:00Z2016 Jun2025-04-26T02:00:35.835Z2019-03-27T02:30:49ZS-EPMC51378442803196610.4155/fsoa-2016-0014