{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Freiberg JA"],"funding":["University of Maryland, Baltimore/University of Maryland, College Park Seed Grant","NIAID NIH HHS","HHS | NIH | National Institute of Allergy and Infectious Diseases"],"pagination":["e00149-16"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5141267"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1(6)"],"pubmed_abstract":["To gain a better understanding of the genes and proteins involved in group A <i>Streptococcus</i> (GAS; <i>Streptococcus pyogenes</i>) biofilm growth, we analyzed the transcriptome, cellular proteome, and cell wall proteome from biofilms at different stages and compared them to those of plankton-stage GAS. Using high-throughput RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) shotgun proteomics, we found distinct expression profiles in the transcriptome and proteome. A total of 46 genes and 41 proteins showed expression across the majority of biofilm time points that was consistently higher or consistently lower than that seen across the majority of planktonic time points. However, there was little overlap between the genes and proteins on these two li"],"journal":["mSystems"],"pubmed_title":["Global Analysis and Comparison of the Transcriptomes and Proteomes of Group A <i>Streptococcus</i> Biofilms."],"pmcid":["PMC5141267"],"funding_grant_id":["R01AI069568","R01AI047928","T32 AI007540","T32AI007540","R01 AI047928"],"pubmed_authors":["Ernst RK","Goodlett DR","Freiberg JA","Scott AJ","Goo YA","McIver KS","Tran BQ","Mongodin EF","Shirtliff ME","Le Breton Y","Harro JM"],"additional_accession":[]},"is_claimable":false,"name":"Global Analysis and Comparison of the Transcriptomes and Proteomes of Group A <i>Streptococcus</i> Biofilms.","description":"To gain a better understanding of the genes and proteins involved in group A <i>Streptococcus</i> (GAS; <i>Streptococcus pyogenes</i>) biofilm growth, we analyzed the transcriptome, cellular proteome, and cell wall proteome from biofilms at different stages and compared them to those of plankton-stage GAS. Using high-throughput RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) shotgun proteomics, we found distinct expression profiles in the transcriptome and proteome. A total of 46 genes and 41 proteins showed expression across the majority of biofilm time points that was consistently higher or consistently lower than that seen across the majority of planktonic time points. However, there was little overlap between the genes and proteins on these two li","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Nov-Dec","modification":"2026-06-10T05:44:20.155Z","creation":"2019-06-06T16:42:51Z"},"accession":"S-EPMC5141267","cross_references":{"pubmed":["27933318"],"doi":["10.1128/mSystems.00149-16"]}}