{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sidobre S"],"funding":["NIAID NIH HHS","Wellcome Trust","NIGMS NIH HHS"],"pagination":["12254-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC514465"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["101(33)"],"pubmed_abstract":["Natural killer (NK) T cells with an invariant Valpha14 rearrangement (Valpha14i) are the largest population of lipid antigen-specific T lymphocytes identified in animals. They react to the glycolipid alpha-galactosyl ceramide (alpha-GalCer) presented by CD1d, and they may have important regulatory functions. It was previously shown that the Valpha14i T cell antigen receptor (TCR) has a high affinity for the alpha-GalCer/CD1d complex, driven by a long half-life (t(1/2)). Although this result could have reflected the unique attributes of alpha-GalCer, using several related glycolipid compounds, we show here that the threshold for full activation of Valpha14i NKT cells by these glycosphingolipids requires a relatively high-affinity TCR interaction with a long t(1/2). Furthermore, our data are"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["The T cell antigen receptor expressed by Valpha14i NKT cells has a unique mode of glycosphingolipid antigen recognition."],"pmcid":["PMC514465"],"funding_grant_id":["R01 AI048933","R01 AI45889","R01 AI48933","R01 AI045053","U54 GM61502","072021/Z/03/Z","R24 GM061502","R01 AI45053","R01 AI045889"],"pubmed_authors":["Hammond KJ","Maltsev SD","Kronenberg M","Ndonye RM","Sakai T","Richardson SK","Howell AR","Sidobre S","Porcelli SA","Benazet-Sidobre L","Besra GS"],"additional_accession":[]},"is_claimable":false,"name":"The T cell antigen receptor expressed by Valpha14i NKT cells has a unique mode of glycosphingolipid antigen recognition.","description":"Natural killer (NK) T cells with an invariant Valpha14 rearrangement (Valpha14i) are the largest population of lipid antigen-specific T lymphocytes identified in animals. They react to the glycolipid alpha-galactosyl ceramide (alpha-GalCer) presented by CD1d, and they may have important regulatory functions. It was previously shown that the Valpha14i T cell antigen receptor (TCR) has a high affinity for the alpha-GalCer/CD1d complex, driven by a long half-life (t(1/2)). Although this result could have reflected the unique attributes of alpha-GalCer, using several related glycolipid compounds, we show here that the threshold for full activation of Valpha14i NKT cells by these glycosphingolipids requires a relatively high-affinity TCR interaction with a long t(1/2). Furthermore, our data are","dates":{"release":"2004-01-01T00:00:00Z","publication":"2004 Aug","modification":"2025-04-05T15:26:54.882Z","creation":"2019-03-27T00:50:42Z"},"accession":"S-EPMC514465","cross_references":{"pubmed":["15304644"],"doi":["10.1073/pnas.0404632101"]}}