{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cottrell KA"],"funding":["National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["e0167877"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5145212"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(12)"],"pubmed_abstract":["Post-transcriptional regulation of gene expression is an important process that is mediated by interactions between mRNAs and RNA binding proteins (RBP), non-coding RNAs (ncRNA) or ribonucleoproteins (RNP). Key to the study of post-transcriptional regulation of mRNAs and the function of ncRNAs such as long non-coding RNAs (lncRNAs) is an understanding of what factors are interacting with these transcripts. While several techniques exist for the enrichment of a transcript whether it is an mRNA or an ncRNA, many of these techniques are cumbersome or limited in their application. Here we present a novel method for the immunoprecipitation of mRNAs and ncRNAs, Urb-RNA immunoprecipitation (Urb-RIP). This method employs the RRM1 domain of the \"resurrected\" snRNA-binding protein Urb to enrich mess"],"journal":["PloS one"],"pubmed_title":["Urb-RIP - An Adaptable and Efficient Approach for Immunoprecipitation of RNAs and Associated RNAs/Proteins."],"pmcid":["PMC5145212"],"funding_grant_id":["GM112824","R01 GM112824","T32 GM007067","GM007067"],"pubmed_authors":["Djuranovic S","Cottrell KA"],"additional_accession":[]},"is_claimable":false,"name":"Urb-RIP - An Adaptable and Efficient Approach for Immunoprecipitation of RNAs and Associated RNAs/Proteins.","description":"Post-transcriptional regulation of gene expression is an important process that is mediated by interactions between mRNAs and RNA binding proteins (RBP), non-coding RNAs (ncRNA) or ribonucleoproteins (RNP). Key to the study of post-transcriptional regulation of mRNAs and the function of ncRNAs such as long non-coding RNAs (lncRNAs) is an understanding of what factors are interacting with these transcripts. While several techniques exist for the enrichment of a transcript whether it is an mRNA or an ncRNA, many of these techniques are cumbersome or limited in their application. Here we present a novel method for the immunoprecipitation of mRNAs and ncRNAs, Urb-RNA immunoprecipitation (Urb-RIP). This method employs the RRM1 domain of the \"resurrected\" snRNA-binding protein Urb to enrich mess","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2025-04-26T14:45:49.857Z","creation":"2019-03-26T22:49:00Z"},"accession":"S-EPMC5145212","cross_references":{"pubmed":["27930710"],"doi":["10.1371/journal.pone.0167877"]}}