biostudies-literatureUnknown7Guo RMicroRNAs (miRNAs) are important for plant development including seed formation, dormancy, and germination, as well as seedling establishment. The <i>Brassica</i> vegetable seedling establishment stage influences the development of high quality seedlings, but also affects the nutrient content of sprouts. Chinese kale (<i>Brassica alboglabra</i>) seedlings at different growth stages were used to construct two small-RNA (sRNA) libraries. We comprehensively analyzed the miRNAs in 2- and 9-day-old seedlings. An average of 11,722,490 clean reads were generated after removing low-quality reads and adapter contaminants. The results revealed that 37.65 and 26.69% of the sRNAs in 2- and 9-day-old seedlings, respectively, were 24 nt long. In total, 254 known mature miRNA sequences from 228 miRNA famFrontiers in plant science1760https://www.ebi.ac.uk/biostudies/studies/S-EPMC5147431biostudies-literatureIdentification of miRNAs Affecting the Establishment of <i>Brassica Alboglabra</i> Seedling.PMC5147431Lai ZHuang ZChen XGuo RDeng YXuHan XfalseIdentification of miRNAs Affecting the Establishment of <i>Brassica Alboglabra</i> Seedling.MicroRNAs (miRNAs) are important for plant development including seed formation, dormancy, and germination, as well as seedling establishment. The <i>Brassica</i> vegetable seedling establishment stage influences the development of high quality seedlings, but also affects the nutrient content of sprouts. Chinese kale (<i>Brassica alboglabra</i>) seedlings at different growth stages were used to construct two small-RNA (sRNA) libraries. We comprehensively analyzed the miRNAs in 2- and 9-day-old seedlings. An average of 11,722,490 clean reads were generated after removing low-quality reads and adapter contaminants. The results revealed that 37.65 and 26.69% of the sRNAs in 2- and 9-day-old seedlings, respectively, were 24 nt long. In total, 254 known mature miRNA sequences from 228 miRNA fam2016-01-01T00:00:00Z20162025-04-26T14:42:13.413Z2019-03-27T02:31:09ZS-EPMC51474312801836610.3389/fpls.2016.01760