biostudies-literatureJensen SMNIH HHSNIGMS NIH HHS2216-2219https://www.ebi.ac.uk/biostudies/studies/S-EPMC5170875biostudies-literatureUnknown17(23)Chemical crosslinking is a versatile tool for the examination of biochemical interactions, in particular host-pathogen interactions. We report the critical first step toward the goal of probing these interactions by the synthesis and use of a new heterobifunctional crosslinker containing a triazabutadiene scaffold. The triazabutadiene is stable to protein conjugation and liberates a reactive aryl diazonium species upon irradiation with 350 nm light. We highlight the use of this technology by modifying the surface of several proteins, including the dengue virus envelope protein.Chembiochem : a European journal of chemical biologyLight-Activated Triazabutadienes for the Modification of a Viral Surface.PMC5170875T32 GM008804S10 OD013237Jewett JCKimani FWJensen SMfalseLight-Activated Triazabutadienes for the Modification of a Viral Surface.Chemical crosslinking is a versatile tool for the examination of biochemical interactions, in particular host-pathogen interactions. We report the critical first step toward the goal of probing these interactions by the synthesis and use of a new heterobifunctional crosslinker containing a triazabutadiene scaffold. The triazabutadiene is stable to protein conjugation and liberates a reactive aryl diazonium species upon irradiation with 350 nm light. We highlight the use of this technology by modifying the surface of several proteins, including the dengue virus envelope protein.2016-01-01T00:00:00Z2016 Dec2026-04-16T17:20:09.292Z2019-03-27T02:31:58ZS-EPMC51708752764778610.1002/cbic.201600508