biostudies-literatureQu YDOE-BEREMSL intramural research projectsEMSL capability development projectsNIEHS NIH HHSNational Institute of Environmental Health Sciences of the National Institutes of HealthU.S. Department of Energy Office of Biological and Environmental ResearchDOE207-215https://www.ebi.ac.uk/biostudies/studies/S-EPMC5175459biostudies-literatureUnknown72Protein glycosylation, an important and complex post-translational modification (PTM), is involved in various biological processes, including the receptor-ligand and cell-cell interaction, and plays a crucial role in many biological functions. However, little is known about the glycan structures of important biological complex samples, and the conventional glycan enrichment strategy (i.e., size-exclusion column [SEC] separation) prior to nuclear magnetic resonance (NMR) detection is time-consuming and tedious. In this study, we developed a glycan enrichment strategy that couples Zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) with dialysis to enrich the glycans from the pronase E digests of RNase B, followed by NMR analysis of the glycoconjugate. Our results suggest Fungal genetics and biology : FG & BStructural analysis of N- and O-glycans using ZIC-HILIC/dialysis coupled to NMR detection.PMC5175459R01ES022176R01 ES022176DE-AC05-76RL01830Cao LZink EMDeng SLipton MSPasa-Tolic LZhang QHu JZJiang YFeng JZhao RZhang ZWu SQu YBaker SEfalseStructural analysis of N- and O-glycans using ZIC-HILIC/dialysis coupled to NMR detection.Protein glycosylation, an important and complex post-translational modification (PTM), is involved in various biological processes, including the receptor-ligand and cell-cell interaction, and plays a crucial role in many biological functions. However, little is known about the glycan structures of important biological complex samples, and the conventional glycan enrichment strategy (i.e., size-exclusion column [SEC] separation) prior to nuclear magnetic resonance (NMR) detection is time-consuming and tedious. In this study, we developed a glycan enrichment strategy that couples Zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) with dialysis to enrich the glycans from the pronase E digests of RNase B, followed by NMR analysis of the glycoconjugate. Our results suggest 2014-01-01T00:00:00Z2014 Nov2025-04-18T23:59:20.908Z2019-03-27T02:32:12ZS-EPMC51754592511769310.1016/j.fgb.2014.08.001