{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Furtauer L"],"funding":["Austrian Science Fund FWF"],"pagination":["1912"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5177628"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7"],"pubmed_abstract":["Although compartmentation is a key feature of eukaryotic cells, biological research is frequently limited by methods allowing for the comprehensive subcellular resolution of the metabolome. It has been widely accepted that such a resolution would be necessary in order to approximate cellular biochemistry and metabolic regulation, yet technical challenges still limit both the reproducible subcellular fractionation and the sample throughput being necessary for a statistically robust analysis. Here, we present a method and a detailed protocol which is based on the non-aqueous fractionation technique enabling the assignment of metabolites to their subcellular localization. The presented benchtop method aims at unraveling subcellular metabolome dynamics in a precise and statistically robust man"],"journal":["Frontiers in plant science"],"pubmed_title":["A Benchtop Fractionation Procedure for Subcellular Analysis of the Plant Metabolome."],"pmcid":["PMC5177628"],"funding_grant_id":["I 2071","I2071"],"pubmed_authors":["Nagele T","Furtauer L","Weckwerth W"],"additional_accession":[]},"is_claimable":false,"name":"A Benchtop Fractionation Procedure for Subcellular Analysis of the Plant Metabolome.","description":"Although compartmentation is a key feature of eukaryotic cells, biological research is frequently limited by methods allowing for the comprehensive subcellular resolution of the metabolome. It has been widely accepted that such a resolution would be necessary in order to approximate cellular biochemistry and metabolic regulation, yet technical challenges still limit both the reproducible subcellular fractionation and the sample throughput being necessary for a statistically robust analysis. Here, we present a method and a detailed protocol which is based on the non-aqueous fractionation technique enabling the assignment of metabolites to their subcellular localization. The presented benchtop method aims at unraveling subcellular metabolome dynamics in a precise and statistically robust man","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2026-04-08T15:53:21.384Z","creation":"2019-03-27T02:32:16Z"},"accession":"S-EPMC5177628","cross_references":{"pubmed":["28066469"],"doi":["10.3389/fpls.2016.01912"]}}