biostudies-literatureFurtauer LAustrian Science Fund FWF1912https://www.ebi.ac.uk/biostudies/studies/S-EPMC5177628biostudies-literatureUnknown7Although compartmentation is a key feature of eukaryotic cells, biological research is frequently limited by methods allowing for the comprehensive subcellular resolution of the metabolome. It has been widely accepted that such a resolution would be necessary in order to approximate cellular biochemistry and metabolic regulation, yet technical challenges still limit both the reproducible subcellular fractionation and the sample throughput being necessary for a statistically robust analysis. Here, we present a method and a detailed protocol which is based on the non-aqueous fractionation technique enabling the assignment of metabolites to their subcellular localization. The presented benchtop method aims at unraveling subcellular metabolome dynamics in a precise and statistically robust manFrontiers in plant scienceA Benchtop Fractionation Procedure for Subcellular Analysis of the Plant Metabolome.PMC5177628I 2071I2071Nagele TFurtauer LWeckwerth WfalseA Benchtop Fractionation Procedure for Subcellular Analysis of the Plant Metabolome.Although compartmentation is a key feature of eukaryotic cells, biological research is frequently limited by methods allowing for the comprehensive subcellular resolution of the metabolome. It has been widely accepted that such a resolution would be necessary in order to approximate cellular biochemistry and metabolic regulation, yet technical challenges still limit both the reproducible subcellular fractionation and the sample throughput being necessary for a statistically robust analysis. Here, we present a method and a detailed protocol which is based on the non-aqueous fractionation technique enabling the assignment of metabolites to their subcellular localization. The presented benchtop method aims at unraveling subcellular metabolome dynamics in a precise and statistically robust man2016-01-01T00:00:00Z20162026-04-08T15:53:21.384Z2019-03-27T02:32:16ZS-EPMC51776282806646910.3389/fpls.2016.01912