biostudies-literatureCorcoran MMNIAID NIH HHS13642https://www.ebi.ac.uk/biostudies/studies/S-EPMC5187446biostudies-literatureUnknown7Comprehensive knowledge of immunoglobulin genetics is required to advance our understanding of B cell biology. Validated immunoglobulin variable (V) gene databases are close to completion only for human and mouse. We present a novel computational approach, IgDiscover, that identifies germline V genes from expressed repertoires to a specificity of 100%. IgDiscover uses a cluster identification process to produce candidate sequences that, once filtered, results in individualized germline V gene databases. IgDiscover was tested in multiple species, validated by genomic cloning and cross library comparisons and produces comprehensive gene databases even where limited genomic sequence is available. IgDiscover analysis of the allelic content of the Indian and Chinese-origin rhesus macaques reveaNature communicationsProduction of individualized V gene databases reveals high levels of immunoglobulin genetic diversity.PMC5187446P01 AI104722Vazquez BernatCorcoran MMStahl-Hennig CSumida NPersson MAMartin MPhad GEKarlsson Hedestam GBfalseProduction of individualized V gene databases reveals high levels of immunoglobulin genetic diversity.Comprehensive knowledge of immunoglobulin genetics is required to advance our understanding of B cell biology. Validated immunoglobulin variable (V) gene databases are close to completion only for human and mouse. We present a novel computational approach, IgDiscover, that identifies germline V genes from expressed repertoires to a specificity of 100%. IgDiscover uses a cluster identification process to produce candidate sequences that, once filtered, results in individualized germline V gene databases. IgDiscover was tested in multiple species, validated by genomic cloning and cross library comparisons and produces comprehensive gene databases even where limited genomic sequence is available. IgDiscover analysis of the allelic content of the Indian and Chinese-origin rhesus macaques revea2016-01-01T00:00:00Z2016 Dec2026-05-02T07:26:20.441Z2019-03-27T02:32:31ZS-EPMC51874462799592810.1038/ncomms13642