{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Alagesan K"],"funding":["Beilstein Institut","Seventh Framework Programme","Max-Planck-Gesellschaft"],"pagination":["529-538"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5203826"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["409(2)"],"pubmed_abstract":["Glycopeptide enrichment is a crucial step in glycoproteomics for which hydrophilic interaction chromatography (HILIC) has extensively been applied due to its low bias towards different glycan types. A systematic evaluation of applicable HILIC mobile phases on glycopeptide enrichment efficiency and selectivity is, to date, however, still lacking. Here, we present a novel, simplified technique for HILIC enrichment termed \"Drop-HILIC\", which was applied to systematically evaluate the mobile phase effect on ZIC-HILIC (zwitterionic type of hydrophilic interaction chromatography) glycopeptide enrichment. The four most commonly used MS compatible organic solvents were investigated: (i) acetonitrile, (ii) methanol, (iii) ethanol and (iv) isopropanol. Glycopeptide enrichment efficiencies were evalu"],"journal":["Analytical and bioanalytical chemistry"],"pubmed_title":["It is all about the solvent: on the importance of the mobile phase for ZIC-HILIC glycopeptide enrichment."],"pmcid":["PMC5203826"],"funding_grant_id":["PhD Scholarship","intramural funds","PCIG09-GA-2011-293847"],"pubmed_authors":["Alagesan K","Kolarich D","Khilji SK"],"additional_accession":[]},"is_claimable":false,"name":"It is all about the solvent: on the importance of the mobile phase for ZIC-HILIC glycopeptide enrichment.","description":"Glycopeptide enrichment is a crucial step in glycoproteomics for which hydrophilic interaction chromatography (HILIC) has extensively been applied due to its low bias towards different glycan types. A systematic evaluation of applicable HILIC mobile phases on glycopeptide enrichment efficiency and selectivity is, to date, however, still lacking. Here, we present a novel, simplified technique for HILIC enrichment termed \"Drop-HILIC\", which was applied to systematically evaluate the mobile phase effect on ZIC-HILIC (zwitterionic type of hydrophilic interaction chromatography) glycopeptide enrichment. The four most commonly used MS compatible organic solvents were investigated: (i) acetonitrile, (ii) methanol, (iii) ethanol and (iv) isopropanol. Glycopeptide enrichment efficiencies were evalu","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jan","modification":"2026-05-02T04:13:12.37Z","creation":"2025-05-29T20:26:35.348Z"},"accession":"S-EPMC5203826","cross_references":{"pubmed":["27909778"],"doi":["10.1007/s00216-016-0051-6"]}}