biostudies-literatureAlagesan KBeilstein InstitutSeventh Framework ProgrammeMax-Planck-Gesellschaft529-538https://www.ebi.ac.uk/biostudies/studies/S-EPMC5203826biostudies-literatureUnknown409(2)Glycopeptide enrichment is a crucial step in glycoproteomics for which hydrophilic interaction chromatography (HILIC) has extensively been applied due to its low bias towards different glycan types. A systematic evaluation of applicable HILIC mobile phases on glycopeptide enrichment efficiency and selectivity is, to date, however, still lacking. Here, we present a novel, simplified technique for HILIC enrichment termed "Drop-HILIC", which was applied to systematically evaluate the mobile phase effect on ZIC-HILIC (zwitterionic type of hydrophilic interaction chromatography) glycopeptide enrichment. The four most commonly used MS compatible organic solvents were investigated: (i) acetonitrile, (ii) methanol, (iii) ethanol and (iv) isopropanol. Glycopeptide enrichment efficiencies were evaluAnalytical and bioanalytical chemistryIt is all about the solvent: on the importance of the mobile phase for ZIC-HILIC glycopeptide enrichment.PMC5203826PhD Scholarshipintramural fundsPCIG09-GA-2011-293847Alagesan KKolarich DKhilji SKfalseIt is all about the solvent: on the importance of the mobile phase for ZIC-HILIC glycopeptide enrichment.Glycopeptide enrichment is a crucial step in glycoproteomics for which hydrophilic interaction chromatography (HILIC) has extensively been applied due to its low bias towards different glycan types. A systematic evaluation of applicable HILIC mobile phases on glycopeptide enrichment efficiency and selectivity is, to date, however, still lacking. Here, we present a novel, simplified technique for HILIC enrichment termed "Drop-HILIC", which was applied to systematically evaluate the mobile phase effect on ZIC-HILIC (zwitterionic type of hydrophilic interaction chromatography) glycopeptide enrichment. The four most commonly used MS compatible organic solvents were investigated: (i) acetonitrile, (ii) methanol, (iii) ethanol and (iv) isopropanol. Glycopeptide enrichment efficiencies were evalu2017-01-01T00:00:00Z2017 Jan2026-05-02T04:13:12.37Z2025-05-29T20:26:35.348ZS-EPMC52038262790977810.1007/s00216-016-0051-6