{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tilwani RK"],"funding":["UM High Impact Research Grant","AO Foundation","QMUL Principal PhD studentship"],"pagination":["49-58"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5209429"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["66(1)"],"pubmed_abstract":["<h4>Objective and design</h4>Oxygen tension and biomechanical signals are factors that regulate inflammatory mechanisms in chondrocytes. We examined whether low oxygen tension influenced the cells response to TNFα and dynamic compression.<h4>Materials and methods</h4>Chondrocyte/agarose constructs were treated with varying concentrations of TNFα (0.1-100 ng/ml) and cultured at 5 and 21 % oxygen tension for 48 h. In separate experiments, constructs were subjected to dynamic compression (15 %) and treated with TNFα (10 ng/ml) and/or L-NIO (1 mM) at 5 and 21 % oxygen tension using an ex vivo bioreactor for 48 h. Markers for catabolic activity (NO, PGE<sub>2</sub>) and tissue remodelling (GAG, MMPs) were quantified by biochemical assay. ADAMTS-5 and MMP-13 expression were examined by real-time"],"journal":["Inflammation research : official journal of the European Histamine Research Society ... [et al.]"],"pubmed_title":["Oxygen tension modulates the effects of TNFα in compressed chondrocytes."],"pmcid":["PMC5209429"],"funding_grant_id":["S-09-83C","UM.C/HIR/MOHE/ENG/44"],"pubmed_authors":["Pingguan-Murphy B","Bader DL","Chowdhury TT","Lee DA","Vessillier S","Tilwani RK"],"additional_accession":[]},"is_claimable":false,"name":"Oxygen tension modulates the effects of TNFα in compressed chondrocytes.","description":"<h4>Objective and design</h4>Oxygen tension and biomechanical signals are factors that regulate inflammatory mechanisms in chondrocytes. We examined whether low oxygen tension influenced the cells response to TNFα and dynamic compression.<h4>Materials and methods</h4>Chondrocyte/agarose constructs were treated with varying concentrations of TNFα (0.1-100 ng/ml) and cultured at 5 and 21 % oxygen tension for 48 h. In separate experiments, constructs were subjected to dynamic compression (15 %) and treated with TNFα (10 ng/ml) and/or L-NIO (1 mM) at 5 and 21 % oxygen tension using an ex vivo bioreactor for 48 h. Markers for catabolic activity (NO, PGE<sub>2</sub>) and tissue remodelling (GAG, MMPs) were quantified by biochemical assay. ADAMTS-5 and MMP-13 expression were examined by real-time","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jan","modification":"2025-04-26T05:39:59.487Z","creation":"2019-06-06T16:49:07Z"},"accession":"S-EPMC5209429","cross_references":{"pubmed":["27658702"],"doi":["10.1007/s00011-016-0991-5"]}}