biostudies-literatureTilwani RKUM High Impact Research GrantAO FoundationQMUL Principal PhD studentship49-58https://www.ebi.ac.uk/biostudies/studies/S-EPMC5209429biostudies-literatureUnknown66(1)<h4>Objective and design</h4>Oxygen tension and biomechanical signals are factors that regulate inflammatory mechanisms in chondrocytes. We examined whether low oxygen tension influenced the cells response to TNFα and dynamic compression.<h4>Materials and methods</h4>Chondrocyte/agarose constructs were treated with varying concentrations of TNFα (0.1-100 ng/ml) and cultured at 5 and 21 % oxygen tension for 48 h. In separate experiments, constructs were subjected to dynamic compression (15 %) and treated with TNFα (10 ng/ml) and/or L-NIO (1 mM) at 5 and 21 % oxygen tension using an ex vivo bioreactor for 48 h. Markers for catabolic activity (NO, PGE₂) and tissue remodelling (GAG, MMPs) were quantified by biochemical assay. ADAMTS-5 and MMP-13 expression were examined by real-timeInflammation research : official journal of the European Histamine Research Society ... [et al.]Oxygen tension modulates the effects of TNFα in compressed chondrocytes.PMC5209429S-09-83CUM.C/HIR/MOHE/ENG/44Pingguan-Murphy BBader DLChowdhury TTLee DAVessillier STilwani RKfalseOxygen tension modulates the effects of TNFα in compressed chondrocytes.<h4>Objective and design</h4>Oxygen tension and biomechanical signals are factors that regulate inflammatory mechanisms in chondrocytes. We examined whether low oxygen tension influenced the cells response to TNFα and dynamic compression.<h4>Materials and methods</h4>Chondrocyte/agarose constructs were treated with varying concentrations of TNFα (0.1-100 ng/ml) and cultured at 5 and 21 % oxygen tension for 48 h. In separate experiments, constructs were subjected to dynamic compression (15 %) and treated with TNFα (10 ng/ml) and/or L-NIO (1 mM) at 5 and 21 % oxygen tension using an ex vivo bioreactor for 48 h. Markers for catabolic activity (NO, PGE₂) and tissue remodelling (GAG, MMPs) were quantified by biochemical assay. ADAMTS-5 and MMP-13 expression were examined by real-time2017-01-01T00:00:00Z2017 Jan2025-04-26T05:39:59.487Z2019-06-06T16:49:07ZS-EPMC52094292765870210.1007/s00011-016-0991-5