{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Ho PW"],"funding":["Deutsche Forschungsgemeinschaft"],"pubmed_abstract":["<h4>Background</h4>Glycerol is an abundant by-product of biodiesel production and has several advantages as a substrate in biotechnological applications. Unfortunately, the popular production host <i>Saccharomyces cerevisiae</i> can barely metabolize glycerol by nature.<h4>Results</h4>In this study, two evolved derivatives of the strain CEN.PK113-1A were created that were able to grow in synthetic glycerol medium (strains PW-1 and PW-2). Their growth performances on glycerol were compared with that of the previously published evolved CEN.PK113-7D derivative JL1. As JL1 showed a higher maximum specific growth rate on glycerol (0.164 h<sup>-1</sup> compared to 0.119 h<sup>-1</sup> for PW-1 and 0.127 h<sup>-1</sup> for PW-2), its genomic DNA was subjected to whole-genome resequencing. Two poi"],"journal":["Biotechnology for biofuels"],"pagination":["10"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5209837"],"repository":["biostudies-literature"],"pubmed_title":["The sole introduction of two single-point mutations establishes glycerol utilization in <i>Saccharomyces cerevisiae</i> CEN.PK derivatives."],"pmcid":["PMC5209837"],"pubmed_authors":["Duitama J","Ho PW","Nevoigt E","Swinnen S"],"additional_accession":[]},"is_claimable":false,"name":"The sole introduction of two single-point mutations establishes glycerol utilization in <i>Saccharomyces cerevisiae</i> CEN.PK derivatives.","description":"<h4>Background</h4>Glycerol is an abundant by-product of biodiesel production and has several advantages as a substrate in biotechnological applications. Unfortunately, the popular production host <i>Saccharomyces cerevisiae</i> can barely metabolize glycerol by nature.<h4>Results</h4>In this study, two evolved derivatives of the strain CEN.PK113-1A were created that were able to grow in synthetic glycerol medium (strains PW-1 and PW-2). Their growth performances on glycerol were compared with that of the previously published evolved CEN.PK113-7D derivative JL1. As JL1 showed a higher maximum specific growth rate on glycerol (0.164 h<sup>-1</sup> compared to 0.119 h<sup>-1</sup> for PW-1 and 0.127 h<sup>-1</sup> for PW-2), its genomic DNA was subjected to whole-genome resequencing. Two poi","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2026-06-08T06:30:09.734Z","creation":"2026-06-08T03:14:17.007Z"},"accession":"S-EPMC5209837","cross_references":{"pubmed":["28053667"],"doi":["10.1186/s13068-016-0696-6"]}}