biostudies-literatureUnknown10Ho PWDeutsche Forschungsgemeinschaft<h4>Background</h4>Glycerol is an abundant by-product of biodiesel production and has several advantages as a substrate in biotechnological applications. Unfortunately, the popular production host <i>Saccharomyces cerevisiae</i> can barely metabolize glycerol by nature.<h4>Results</h4>In this study, two evolved derivatives of the strain CEN.PK113-1A were created that were able to grow in synthetic glycerol medium (strains PW-1 and PW-2). Their growth performances on glycerol were compared with that of the previously published evolved CEN.PK113-7D derivative JL1. As JL1 showed a higher maximum specific growth rate on glycerol (0.164 h^-1 compared to 0.119 h^-1 for PW-1 and 0.127 h^-1 for PW-2), its genomic DNA was subjected to whole-genome resequencing. Two poiBiotechnology for biofuels10https://www.ebi.ac.uk/biostudies/studies/S-EPMC5209837biostudies-literatureThe sole introduction of two single-point mutations establishes glycerol utilization in <i>Saccharomyces cerevisiae</i> CEN.PK derivatives.PMC5209837Duitama JHo PWNevoigt ESwinnen SfalseThe sole introduction of two single-point mutations establishes glycerol utilization in <i>Saccharomyces cerevisiae</i> CEN.PK derivatives.<h4>Background</h4>Glycerol is an abundant by-product of biodiesel production and has several advantages as a substrate in biotechnological applications. Unfortunately, the popular production host <i>Saccharomyces cerevisiae</i> can barely metabolize glycerol by nature.<h4>Results</h4>In this study, two evolved derivatives of the strain CEN.PK113-1A were created that were able to grow in synthetic glycerol medium (strains PW-1 and PW-2). Their growth performances on glycerol were compared with that of the previously published evolved CEN.PK113-7D derivative JL1. As JL1 showed a higher maximum specific growth rate on glycerol (0.164 h^-1 compared to 0.119 h^-1 for PW-1 and 0.127 h^-1 for PW-2), its genomic DNA was subjected to whole-genome resequencing. Two poi2017-01-01T00:00:00Z20172026-06-08T06:30:09.734Z2026-06-08T03:14:17.007ZS-EPMC52098372805366710.1186/s13068-016-0696-6