biostudies-literature00440Unknown7(30)Sablin MP<h4>Background</h4>We aimed at identifying druggable molecular alterations at the RNA level from untreated HNSCC patients, and assessing their prognostic significance.<h4>Methods</h4>We retrieved 96 HNSCC patients who underwent primary surgery. Real-time quantitative RT-PCR was used to analyze a panel of 42 genes coding for major druggable proteins. Univariate and multivariate analyses were performed to assess the prognostic significance of overexpressed genes.<h4>Results</h4>Median age was 56 years [35-78]. Most of patients were men (80%) with a history of alcohol (70.4%) and/or tobacco consumption (72.5%). Twelve patients (12%) were HPV-positive. Most significantly overexpressed genes involved cell cycle regulation (CCND1 [27%], CDK6 [21%]), tyrosine kinase receptors (MET [18%], EGFR [14%]), angiogenesis (PGF [301%], VEGFA [14%]), and immune system (PDL1/CD274 [28%]). PIK3CA expression was an independent prognostic marker, associated with shorter disease-free survival.<h4>Conclusions</h4>We identified druggable overexpressed genes associated with a poor outcome that might be of interest for personalizing treatment of HNSCC patients.Oncotarget47418-47430https://www.ebi.ac.uk/biostudies/studies/S-EPMC5216951biostudies-literatureIdentification of new candidate therapeutic target genes in head and neck squamous cell carcinomas.PMC5216951Sastre-Garau XMariani OCaly MRodriguez JGirod AKamal MLappartient EVacher SSablin MPHoffmann CBerger FChemlali WOuafi LBieche IKlijanienko JJouffroy TCalugaru VLe Tourneau CLidereau RDubot C44falseIdentification of new candidate therapeutic target genes in head and neck squamous cell carcinomas.<h4>Background</h4>We aimed at identifying druggable molecular alterations at the RNA level from untreated HNSCC patients, and assessing their prognostic significance.<h4>Methods</h4>We retrieved 96 HNSCC patients who underwent primary surgery. Real-time quantitative RT-PCR was used to analyze a panel of 42 genes coding for major druggable proteins. Univariate and multivariate analyses were performed to assess the prognostic significance of overexpressed genes.<h4>Results</h4>Median age was 56 years [35-78]. Most of patients were men (80%) with a history of alcohol (70.4%) and/or tobacco consumption (72.5%). Twelve patients (12%) were HPV-positive. Most significantly overexpressed genes involved cell cycle regulation (CCND1 [27%], CDK6 [21%]), tyrosine kinase receptors (MET [18%], EGFR [14%]), angiogenesis (PGF [301%], VEGFA [14%]), and immune system (PDL1/CD274 [28%]). PIK3CA expression was an independent prognostic marker, associated with shorter disease-free survival.<h4>Conclusions</h4>We identified druggable overexpressed genes associated with a poor outcome that might be of interest for personalizing treatment of HNSCC patients.2016-01-01T00:00:00Z2016 Jul2024-11-20T10:06:50.129Z2019-03-27T02:33:24ZS-EPMC52169512732972610.18632/oncotarget.10163