biostudies-literatureLateef ZHealth Research Council of New Zealand39https://www.ebi.ac.uk/biostudies/studies/S-EPMC5217272biostudies-literatureUnknown18(1)<h4>Background</h4>The GII.4 Sydney 2012 strain of human norovirus (HuNoV) is a pandemic strain that is responsible for the majority of norovirus outbreaks in healthcare settings. The function of the non-structural (NS)1-2 protein from HuNoV is unknown.<h4>Results</h4>In silico analysis of human norovirus NS1-2 protein showed that it shares features with the murine NS1-2 protein, including a disordered region, a transmembrane domain and H-box and NC sequence motifs. The proteins also contain caspase cleavage and phosphorylation sites, indicating that processing and phosphorylation may be a conserved feature of norovirus NS1-2 proteins. In this study, RNA transcripts of human and murine norovirus full-length and the disordered region of NS1-2 were transfected into monocytes, and next generaBMC genomicsTranscriptomic analysis of human norovirus NS1-2 protein highlights a multifunctional role in murine monocytes.PMC521727212-057 Career Development AwardBaker ESWard VKGimenez GLateef ZfalseTranscriptomic analysis of human norovirus NS1-2 protein highlights a multifunctional role in murine monocytes.<h4>Background</h4>The GII.4 Sydney 2012 strain of human norovirus (HuNoV) is a pandemic strain that is responsible for the majority of norovirus outbreaks in healthcare settings. The function of the non-structural (NS)1-2 protein from HuNoV is unknown.<h4>Results</h4>In silico analysis of human norovirus NS1-2 protein showed that it shares features with the murine NS1-2 protein, including a disordered region, a transmembrane domain and H-box and NC sequence motifs. The proteins also contain caspase cleavage and phosphorylation sites, indicating that processing and phosphorylation may be a conserved feature of norovirus NS1-2 proteins. In this study, RNA transcripts of human and murine norovirus full-length and the disordered region of NS1-2 were transfected into monocytes, and next genera2017-01-01T00:00:00Z2017 Jan2026-06-10T05:43:32.76Z2019-06-06T16:50:15ZS-EPMC52172722805677310.1186/s12864-016-3417-4