{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mattes TA"],"funding":["National Institutes of Natural Sciences","U.S. Public Health Service","NIGMS NIH HHS"],"pagination":["269-281"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5218876"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["103(2)"],"pubmed_abstract":["5,6-Dimethylbenzimidazolyl-(DMB)-α-ribotide [α-ribazole-5'-phosphate (α-RP)] is an intermediate in the biosynthesis of adenosylcobalamin (AdoCbl) in many prokaryotes. In such microbes, α-RP is synthesized by nicotinate mononucleotide (NaMN):DMB phosphoribosyltransferases (CobT in Salmonella enterica), in a reaction that is considered to be the canonical step for the activation of the base of the nucleotide present in adenosylcobamides. Some Firmicutes lack CobT-type enzymes but have a two-protein system comprised of a transporter (i.e., CblT) and a kinase (i.e., CblS) that can salvage exogenous α-ribazole (α-R) from the environment using CblT to take up α-R, followed by α-R phosphorylation by CblS. We report that Geobacillus kaustophilus CblT and CblS proteins restore α-RP synthesis in S. "],"journal":["Molecular microbiology"],"pubmed_title":["Salmonella enterica synthesizes 5,6-dimethylbenzimidazolyl-(DMB)-α-riboside. Why some Firmicutes do not require the canonical DMB activation system to synthesize adenosylcobalamin."],"pmcid":["PMC5218876"],"funding_grant_id":["R37 GM040313","R37 GM40313"],"pubmed_authors":["Escalante-Semerena JC","Mattes TA"],"additional_accession":[]},"is_claimable":false,"name":"Salmonella enterica synthesizes 5,6-dimethylbenzimidazolyl-(DMB)-α-riboside. Why some Firmicutes do not require the canonical DMB activation system to synthesize adenosylcobalamin.","description":"5,6-Dimethylbenzimidazolyl-(DMB)-α-ribotide [α-ribazole-5'-phosphate (α-RP)] is an intermediate in the biosynthesis of adenosylcobalamin (AdoCbl) in many prokaryotes. In such microbes, α-RP is synthesized by nicotinate mononucleotide (NaMN):DMB phosphoribosyltransferases (CobT in Salmonella enterica), in a reaction that is considered to be the canonical step for the activation of the base of the nucleotide present in adenosylcobamides. Some Firmicutes lack CobT-type enzymes but have a two-protein system comprised of a transporter (i.e., CblT) and a kinase (i.e., CblS) that can salvage exogenous α-ribazole (α-R) from the environment using CblT to take up α-R, followed by α-R phosphorylation by CblS. We report that Geobacillus kaustophilus CblT and CblS proteins restore α-RP synthesis in S. ","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jan","modification":"2025-04-04T01:31:33.95Z","creation":"2019-03-27T02:33:32Z"},"accession":"S-EPMC5218876","cross_references":{"pubmed":["27748967"],"doi":["10.1111/mmi.13555"]}}