{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["van Wijk XM"],"funding":["Novo Nordisk Foundation","HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","HHS | NIH | National Institute of General Medical Sciences","NIDDK NIH HHS","NHLBI NIH HHS","HHS | NIH | National Heart, Lung, and Blood Institute","NNF Center for Biosustainability","Novo Nordisk Fonden","Wellcome Trust","NIGMS NIH HHS"],"pagination":["e02128-16"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5225314"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["To understand the role of glycosaminoglycans in bacterial cellular invasion, xylosyltransferase-deficient mutants of Chinese hamster ovary (CHO) cells were created using clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated gene 9 (CRISPR-cas9) gene targeting. When these mutants were compared to the pgsA745 cell line, a CHO xylosyltransferase mutant generated previously using chemical mutagenesis, an unexpected result was obtained. Bacterial invasion of pgsA745 cells by group B Streptococcus (GBS), group A Streptococcus, and Staphylococcus aureus was markedly reduced compared to the invasion of wild-type cells, but newly generated CRISPR-cas9 mutants were only resistant to GBS. Invasion of pgsA745 cells was not restored by transfection with xylosyltransfer"],"journal":["mBio"],"pubmed_title":["Whole-Genome Sequencing of Invasion-Resistant Cells Identifies Laminin α2 as a Host Factor for Bacterial Invasion."],"pmcid":["PMC5225314"],"funding_grant_id":["R01 DK036425","CC2185","GM33063","R37 GM033063","DK36425","P01 HL131474","NNF10CC1016517","R37 DK036425","Genome Scale CHO in Silico Models","P01 HL107150","CHO in Silico Protein Quality Engin","NNF16CC002185","HL107150","R01 GM033063","Network Reconstruction","NNF10CC10165178"],"pubmed_authors":["Dohrmann S","Esko JD","Palsson BO","Voldborg BG","Meng BX","Li S","Lewis NE","McKee KK","van Wijk XM","van Kuppevelt TH","Yurchenco PD","Hallstrom BM","Nizet V"],"additional_accession":[]},"is_claimable":false,"name":"Whole-Genome Sequencing of Invasion-Resistant Cells Identifies Laminin α2 as a Host Factor for Bacterial Invasion.","description":"To understand the role of glycosaminoglycans in bacterial cellular invasion, xylosyltransferase-deficient mutants of Chinese hamster ovary (CHO) cells were created using clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated gene 9 (CRISPR-cas9) gene targeting. When these mutants were compared to the pgsA745 cell line, a CHO xylosyltransferase mutant generated previously using chemical mutagenesis, an unexpected result was obtained. Bacterial invasion of pgsA745 cells by group B Streptococcus (GBS), group A Streptococcus, and Staphylococcus aureus was markedly reduced compared to the invasion of wild-type cells, but newly generated CRISPR-cas9 mutants were only resistant to GBS. Invasion of pgsA745 cells was not restored by transfection with xylosyltransfer","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jan","modification":"2026-06-07T08:44:00.58Z","creation":"2026-06-07T03:12:41.496Z"},"accession":"S-EPMC5225314","cross_references":{"pubmed":["28074024"],"doi":["10.1128/mBio.02128-16"]}}