biostudies-literaturevan Wijk XMNovo Nordisk FoundationHHS | NIH | National Institute of Diabetes and Digestive and Kidney DiseasesHHS | NIH | National Institute of General Medical SciencesNIDDK NIH HHSNHLBI NIH HHSHHS | NIH | National Heart, Lung, and Blood InstituteNNF Center for BiosustainabilityNovo Nordisk FondenWellcome TrustNIGMS NIH HHSe02128-16https://www.ebi.ac.uk/biostudies/studies/S-EPMC5225314biostudies-literatureUnknown8(1)To understand the role of glycosaminoglycans in bacterial cellular invasion, xylosyltransferase-deficient mutants of Chinese hamster ovary (CHO) cells were created using clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated gene 9 (CRISPR-cas9) gene targeting. When these mutants were compared to the pgsA745 cell line, a CHO xylosyltransferase mutant generated previously using chemical mutagenesis, an unexpected result was obtained. Bacterial invasion of pgsA745 cells by group B Streptococcus (GBS), group A Streptococcus, and Staphylococcus aureus was markedly reduced compared to the invasion of wild-type cells, but newly generated CRISPR-cas9 mutants were only resistant to GBS. Invasion of pgsA745 cells was not restored by transfection with xylosyltransfermBioWhole-Genome Sequencing of Invasion-Resistant Cells Identifies Laminin α2 as a Host Factor for Bacterial Invasion.PMC5225314R01 DK036425CC2185GM33063R37 GM033063DK36425P01 HL131474NNF10CC1016517R37 DK036425Genome Scale CHO in Silico ModelsP01 HL107150CHO in Silico Protein Quality EnginNNF16CC002185HL107150R01 GM033063Network ReconstructionNNF10CC10165178Dohrmann SEsko JDPalsson BOVoldborg BGMeng BXLi SLewis NEMcKee KKvan Wijk XMvan Kuppevelt THYurchenco PDHallstrom BMNizet VfalseWhole-Genome Sequencing of Invasion-Resistant Cells Identifies Laminin α2 as a Host Factor for Bacterial Invasion.To understand the role of glycosaminoglycans in bacterial cellular invasion, xylosyltransferase-deficient mutants of Chinese hamster ovary (CHO) cells were created using clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated gene 9 (CRISPR-cas9) gene targeting. When these mutants were compared to the pgsA745 cell line, a CHO xylosyltransferase mutant generated previously using chemical mutagenesis, an unexpected result was obtained. Bacterial invasion of pgsA745 cells by group B Streptococcus (GBS), group A Streptococcus, and Staphylococcus aureus was markedly reduced compared to the invasion of wild-type cells, but newly generated CRISPR-cas9 mutants were only resistant to GBS. Invasion of pgsA745 cells was not restored by transfection with xylosyltransfer2017-01-01T00:00:00Z2017 Jan2026-06-07T08:44:00.58Z2026-06-07T03:12:41.496ZS-EPMC52253142807402410.1128/mBio.02128-16