{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23(19)"],"submitter":["Monferran S"],"pubmed_abstract":["The Ku heterodimer (Ku70/Ku80) plays a central role in DNA double-strand breaks repair. Ku is also expressed on the cell surface of different types of cells where its function remains poorly understood. From a yeast two-hybrid screen, we have identified a specific interaction between the core region of Ku80 and the hemopexin domain of metalloproteinase 9 (MMP-9), a key enzyme involved in the degradation of extracellular matrix (ECM) components. Ku associates with MMP-9 on the surface of leukemic cells as demonstrated by co-immunoprecipitation experiments in membrane extracts and double-label immunofluorescence studies. In normal and tumoral migratory cells, Ku80 and MMP-9 colocalize at the periphery of leading edge of cells and cellular invasion of collagen IV matrices was blocked by antib"],"journal":["The EMBO journal"],"pagination":["3758-68"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC522801"],"repository":["biostudies-literature"],"pubmed_title":["The membrane form of the DNA repair protein Ku interacts at the cell surface with metalloproteinase 9."],"pmcid":["PMC522801"],"pubmed_authors":["Paupert J","Salles B","Monferran S","Muller C","Dauvillier S"],"additional_accession":[]},"is_claimable":false,"name":"The membrane form of the DNA repair protein Ku interacts at the cell surface with metalloproteinase 9.","description":"The Ku heterodimer (Ku70/Ku80) plays a central role in DNA double-strand breaks repair. Ku is also expressed on the cell surface of different types of cells where its function remains poorly understood. From a yeast two-hybrid screen, we have identified a specific interaction between the core region of Ku80 and the hemopexin domain of metalloproteinase 9 (MMP-9), a key enzyme involved in the degradation of extracellular matrix (ECM) components. Ku associates with MMP-9 on the surface of leukemic cells as demonstrated by co-immunoprecipitation experiments in membrane extracts and double-label immunofluorescence studies. In normal and tumoral migratory cells, Ku80 and MMP-9 colocalize at the periphery of leading edge of cells and cellular invasion of collagen IV matrices was blocked by antib","dates":{"release":"2004-01-01T00:00:00Z","publication":"2004 Oct","modification":"2025-05-31T23:06:29.586Z","creation":"2025-05-31T23:06:29.586Z"},"accession":"S-EPMC522801","cross_references":{"pubmed":["15385961"],"doi":["10.1038/sj.emboj.7600403"]}}