biostudies-literatureUnknown12(6)Khaghanzadeh NUmbelliprenin (Umb), a natural coumarin, has demonstrated anti-tumor activities, both <i>in vitro</i> and particularly <i>in vivo</i>, in several types of cancer, including lung cancer. The present study aimed to identify molecular targets of Umb using a high-throughput approach. Lung cancer cell lines, QU-DB (large-cell lung carcinoma) and A549 (adenocarcinoma), were treated with Umb. Differentially-expressed proteins were identified using two-dimensional electrophoresis coupled to mass spectrometry. In the QU-DB cells, differential expression of proteins, including downregulation of the tumorigenic protein heat shock protein 90 kDa and upregulation of the potential anti-tumor proteins Nipsnap1 and glycine-tRNA ligase (GRS), suggested that Umb is a strong anti-tumor compound. In the A549 Oncology letters5295-5302https://www.ebi.ac.uk/biostudies/studies/S-EPMC5228441biostudies-literatureImmune-associated proteins with potential <i>in vivo</i> anti-tumor activities are upregulated in lung cancer cells treated with umbelliprenin: A proteomic approach.PMC5228441Khaghanzadeh NKuramitsu YGhaderi AMojtahedi ZNakamura KfalseImmune-associated proteins with potential <i>in vivo</i> anti-tumor activities are upregulated in lung cancer cells treated with umbelliprenin: A proteomic approach.Umbelliprenin (Umb), a natural coumarin, has demonstrated anti-tumor activities, both <i>in vitro</i> and particularly <i>in vivo</i>, in several types of cancer, including lung cancer. The present study aimed to identify molecular targets of Umb using a high-throughput approach. Lung cancer cell lines, QU-DB (large-cell lung carcinoma) and A549 (adenocarcinoma), were treated with Umb. Differentially-expressed proteins were identified using two-dimensional electrophoresis coupled to mass spectrometry. In the QU-DB cells, differential expression of proteins, including downregulation of the tumorigenic protein heat shock protein 90 kDa and upregulation of the potential anti-tumor proteins Nipsnap1 and glycine-tRNA ligase (GRS), suggested that Umb is a strong anti-tumor compound. In the A549 2016-01-01T00:00:00Z2016 Dec2025-04-27T00:54:03.859Z2021-02-20T07:28:28ZS-EPMC52284412810523810.3892/ol.2016.5352