biostudies-literatureUnknown186(21)Choy WKAssembly of bacterial flagella is developmentally important during both planktonic cell growth and biofilm formation. Flagellar biogenesis is complex, requiring coordinated expression of over 40 genes, and normally commences during the log-to-stationary transition phase. We describe here a novel membrane-localized regulator, MorA, that controls the timing of flagellar development and affects motility, chemotaxis, and biofilm formation in Pseudomonas putida. MorA is conserved among diverse Pseudomonas species, and homologues are present in all Pseudomonas genomes sequenced thus far. In P. putida, the absence of MorA derepresses flagellar development, which leads to constitutive formation of flagella in the mutant cells in all growth phases. In Pseudomonas aeruginosa, the absence of MorA ledJournal of bacteriology7221-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC523210biostudies-literatureMorA defines a new class of regulators affecting flagellar development and biofilm formation in diverse Pseudomonas species.PMC523210Zhou LZhang LHChoy WKSwarup SSyn CKfalseMorA defines a new class of regulators affecting flagellar development and biofilm formation in diverse Pseudomonas species.Assembly of bacterial flagella is developmentally important during both planktonic cell growth and biofilm formation. Flagellar biogenesis is complex, requiring coordinated expression of over 40 genes, and normally commences during the log-to-stationary transition phase. We describe here a novel membrane-localized regulator, MorA, that controls the timing of flagellar development and affects motility, chemotaxis, and biofilm formation in Pseudomonas putida. MorA is conserved among diverse Pseudomonas species, and homologues are present in all Pseudomonas genomes sequenced thus far. In P. putida, the absence of MorA derepresses flagellar development, which leads to constitutive formation of flagella in the mutant cells in all growth phases. In Pseudomonas aeruginosa, the absence of MorA led2004-01-01T00:00:00Z2004 Nov2025-04-27T00:40:41.494Z2019-03-27T01:08:16ZS-EPMC5232101548943310.1128/JB.186.21.7221-7228.200410.1128/jb.186.21.7221-7228.2004