{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kroeger KM"],"funding":["NIEHS NIH HHS","NIGMS NIH HHS"],"pagination":["5480-5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC524285"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["32(18)"],"pubmed_abstract":["Apurinic/apyrimidinic (AP) sites are alkali labile lesions that, when encountered during DNA replication, can block polymerases or potentially result in mutagenic events. Owing to the instability of 2-deoxyribose lesions (AP), a chemically stable tetrahydrofuran analog (F) is often used as a model of abasic sites. A comparison of the two lesions in Saccharomyces cerevisiae revealed that the model lesion and 2-deoxyribose have distinct in vivo effects. Comprehensive comparative analyses of F and AP have not been carried out in Escherichia coli. We conducted a side-by-side investigation of F and AP in E.coli to compare their biological effects and interactions with SOS polymerases. Both lesions were examined in SOS-induced and uninduced cells. Our studies reveal that in uninduced E.coli the "],"journal":["Nucleic acids research"],"pubmed_title":["A comprehensive comparison of DNA replication past 2-deoxyribose and its tetrahydrofuran analog in Escherichia coli."],"pmcid":["PMC524285"],"funding_grant_id":["GM-063028","ES-012259","R01 GM063028","R01 ES012259"],"pubmed_authors":["Kroeger KM","Goodman MF","Greenberg MM"],"additional_accession":[]},"is_claimable":false,"name":"A comprehensive comparison of DNA replication past 2-deoxyribose and its tetrahydrofuran analog in Escherichia coli.","description":"Apurinic/apyrimidinic (AP) sites are alkali labile lesions that, when encountered during DNA replication, can block polymerases or potentially result in mutagenic events. Owing to the instability of 2-deoxyribose lesions (AP), a chemically stable tetrahydrofuran analog (F) is often used as a model of abasic sites. A comparison of the two lesions in Saccharomyces cerevisiae revealed that the model lesion and 2-deoxyribose have distinct in vivo effects. Comprehensive comparative analyses of F and AP have not been carried out in Escherichia coli. We conducted a side-by-side investigation of F and AP in E.coli to compare their biological effects and interactions with SOS polymerases. Both lesions were examined in SOS-induced and uninduced cells. Our studies reveal that in uninduced E.coli the ","dates":{"release":"2004-01-01T00:00:00Z","publication":"2004","modification":"2025-05-31T23:09:45.952Z","creation":"2025-05-31T23:09:45.952Z"},"accession":"S-EPMC524285","cross_references":{"pubmed":["15477395"],"doi":["10.1093/nar/gkh873"]}}