biostudies-literatureSun RNational Natural Science Foundation of China63https://www.ebi.ac.uk/biostudies/studies/S-EPMC5263148biostudies-literatureUnknown8Coconut (<i>Cocos nucifera</i> L.) is a key tropical crop and a member of the monocotyledonous family Arecaceae (<i>Palmaceae</i>). Few genes and related metabolic processes involved in coconut endosperm development have been investigated. In this study, a new member of the <i>WRI1</i> gene family was isolated from coconut endosperm and was named <i>CoWRI1</i>. Its transcriptional activities and interactions with the acetyl-CoA carboxylase (<i>BCCP2</i>) promoter of <i>CoWRI1</i> were confirmed by the yeast two-hybrid and yeast one-hybrid approaches, respectively. Functional characterization was carried out through seed-specific expression in <i>Arabidopsis</i> and endosperm-specific expression in rice. In transgenic <i>Arabidopsis</i>, high over-expressions of <i>CoWRI1</i> in seven indepFrontiers in plant scienceCharacterization and Ectopic Expression of <i>CoWRI1</i>, an AP2/EREBP Domain-Containing Transcription Factor from Coconut (<i>Cocos nucifera</i> L.) Endosperm, Changes the Seeds Oil Content in Transgenic <i>Arabidopsis thaliana</i> and Rice (<i>Oryza sativa</i> L.).PMC526314831160171, 31260193, 31360476, 31460213Zheng YWang RMao TLi DSun RYe RGao LLin YZhang LfalseCharacterization and Ectopic Expression of <i>CoWRI1</i>, an AP2/EREBP Domain-Containing Transcription Factor from Coconut (<i>Cocos nucifera</i> L.) Endosperm, Changes the Seeds Oil Content in Transgenic <i>Arabidopsis thaliana</i> and Rice (<i>Oryza sativa</i> L.).Coconut (<i>Cocos nucifera</i> L.) is a key tropical crop and a member of the monocotyledonous family Arecaceae (<i>Palmaceae</i>). Few genes and related metabolic processes involved in coconut endosperm development have been investigated. In this study, a new member of the <i>WRI1</i> gene family was isolated from coconut endosperm and was named <i>CoWRI1</i>. Its transcriptional activities and interactions with the acetyl-CoA carboxylase (<i>BCCP2</i>) promoter of <i>CoWRI1</i> were confirmed by the yeast two-hybrid and yeast one-hybrid approaches, respectively. Functional characterization was carried out through seed-specific expression in <i>Arabidopsis</i> and endosperm-specific expression in rice. In transgenic <i>Arabidopsis</i>, high over-expressions of <i>CoWRI1</i> in seven indep2017-01-01T00:00:00Z20172025-04-18T23:21:43.898Z2019-06-06T16:54:40ZS-EPMC52631482817991110.3389/fpls.2017.00063