biostudies-literatureBaker JANIAAA NIH HHSNational Institutes of Health139-151https://www.ebi.ac.uk/biostudies/studies/S-EPMC5270764biostudies-literatureUnknown58Alcohol abuse is a complex disorder, which is confounded by other factors, including stress. In the present study, we examined gene expression in the hippocampus of BXD recombinant inbred mice after exposure to ethanol (NOE), stress (RSS), and the combination of both (RSE). Mice were given an intraperitoneal (i.p.) injection of 1.8 g/kg ethanol or saline, and subsets of both groups were exposed to acute restraint stress for 15 min or controls. Gene expression in the hippocampus was examined using microarray analysis. Genes that were significantly (p < 0.05, q < 0.1) differentially expressed were further evaluated. Bioinformatic analyses were predominantly performed using tools available at GeneNetwork.org, and included gene ontology, presence of cis-regulation or polymorphisms, phenotype cAlcohol (Fayetteville, N.Y.)Analyses of differentially expressed genes after exposure to acute stress, acute ethanol, or a combination of both in mice.PMC5270764U01 AA014425R01 AA021951R01AA021951Cook MNLu LLi JYang MMulligan MKZhou DJones BCBaker JAHamre KMfalseAnalyses of differentially expressed genes after exposure to acute stress, acute ethanol, or a combination of both in mice.Alcohol abuse is a complex disorder, which is confounded by other factors, including stress. In the present study, we examined gene expression in the hippocampus of BXD recombinant inbred mice after exposure to ethanol (NOE), stress (RSS), and the combination of both (RSE). Mice were given an intraperitoneal (i.p.) injection of 1.8 g/kg ethanol or saline, and subsets of both groups were exposed to acute restraint stress for 15 min or controls. Gene expression in the hippocampus was examined using microarray analysis. Genes that were significantly (p < 0.05, q < 0.1) differentially expressed were further evaluated. Bioinformatic analyses were predominantly performed using tools available at GeneNetwork.org, and included gene ontology, presence of cis-regulation or polymorphisms, phenotype c2017-01-01T00:00:00Z2017 Feb2026-04-14T19:08:07.916Z2019-03-26T22:59:15ZS-EPMC52707642802785210.1016/j.alcohol.2016.08.008