{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Singh M"],"funding":["Ministry of Education, Culture, Sports, Science, and Technology"],"pagination":["e01154-16"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5278750"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["61(2)"],"pubmed_abstract":["The mechanisms underlying bacterial tolerance to antibiotics are unclear. A possible adaptation strategy was explored by exposure of drug-naive methicillin-susceptible Staphylococcus aureus strain FDA209P to vancomycin in vitro Strains surviving vancomycin treatment (vancomycin survivor strains), which appeared after 96 h of exposure, were slow-growing derivatives of the parent strain. Although the vancomycin MICs for the survivor strains were within the susceptible range, the cytokilling effects of vancomycin at 20-fold the MIC were significantly lower for the survivor strains than for the parent strain. Whole-genome sequencing demonstrated that ileS, encoding isoleucyl-tRNA synthetase (IleRS), was mutated in two of the three vancomycin survivor strains. The IleRS Y723H mutation is locate"],"journal":["Antimicrobial agents and chemotherapy"],"pubmed_title":["In Vitro Tolerance of Drug-Naive Staphylococcus aureus Strain FDA209P to Vancomycin."],"pmcid":["PMC5278750"],"funding_grant_id":["S1201013"],"pubmed_authors":["Hishinuma T","Morimoto Y","Singh M","Sasaki T","Matsuo M","Hiramatsu K"],"additional_accession":[]},"is_claimable":false,"name":"In Vitro Tolerance of Drug-Naive Staphylococcus aureus Strain FDA209P to Vancomycin.","description":"The mechanisms underlying bacterial tolerance to antibiotics are unclear. A possible adaptation strategy was explored by exposure of drug-naive methicillin-susceptible Staphylococcus aureus strain FDA209P to vancomycin in vitro Strains surviving vancomycin treatment (vancomycin survivor strains), which appeared after 96 h of exposure, were slow-growing derivatives of the parent strain. Although the vancomycin MICs for the survivor strains were within the susceptible range, the cytokilling effects of vancomycin at 20-fold the MIC were significantly lower for the survivor strains than for the parent strain. Whole-genome sequencing demonstrated that ileS, encoding isoleucyl-tRNA synthetase (IleRS), was mutated in two of the three vancomycin survivor strains. The IleRS Y723H mutation is locate","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Feb","modification":"2026-05-05T07:40:08.913Z","creation":"2019-03-27T02:35:14Z"},"accession":"S-EPMC5278750","cross_references":{"pubmed":["27855063"],"doi":["10.1128/AAC.01154-16"]}}