biostudies-literatureCole AENIGMS NIH HHS577-588https://www.ebi.ac.uk/biostudies/studies/S-EPMC5289838biostudies-literatureUnknown205(2)While most missense suppressors have very narrow specificities and only suppress the allele against which they were isolated, the sumA missense suppressor from Salmonella enterica serovar Typhimurium is a promiscuous or broad-acting missense suppressor that suppresses numerous missense mutants. The sumA missense suppressor was identified as a glyV tRNA Gly3(GAU/C) missense suppressor that can recognize GAU or GAC aspartic acid codons and insert a glycine amino acid instead of aspartic acid. In addition to rescuing missense mutants caused by glycine to aspartic acid changes as expected, sumA could also rescue a number of other missense mutants as well by changing a neighboring (contacting) aspartic acid to glycine, which compensated for the other amino acid change. Thus the ability of sumA GeneticsThe Promiscuous sumA Missense Suppressor from Salmonella enterica Has an Intriguing Mechanism of Action.PMC5289838R01 GM027068Cole AEHani FMMeservy MAltman ERoth JRAltman RfalseThe Promiscuous sumA Missense Suppressor from Salmonella enterica Has an Intriguing Mechanism of Action.While most missense suppressors have very narrow specificities and only suppress the allele against which they were isolated, the sumA missense suppressor from Salmonella enterica serovar Typhimurium is a promiscuous or broad-acting missense suppressor that suppresses numerous missense mutants. The sumA missense suppressor was identified as a glyV tRNA Gly3(GAU/C) missense suppressor that can recognize GAU or GAC aspartic acid codons and insert a glycine amino acid instead of aspartic acid. In addition to rescuing missense mutants caused by glycine to aspartic acid changes as expected, sumA could also rescue a number of other missense mutants as well by changing a neighboring (contacting) aspartic acid to glycine, which compensated for the other amino acid change. Thus the ability of sumA 2017-01-01T00:00:00Z2017 Feb2025-04-19T23:24:07.078Z2019-03-26T22:59:36ZS-EPMC52898382797449710.1534/genetics.116.196550