{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(1)"],"submitter":["da Silva IA"],"pubmed_abstract":["A major drawback of radiotherapy is the accelerated growth of the surviving tumor cells. Radiotherapy generates a variety of lipids that bind to the receptor for platelet-activating factor, expressed by cells in the tumor microenvironment. In the present study, using the TC-1 tumor cell line, we found that irradiation induced a twofold increase in receptor expression and generated agonists of receptor. Irradiated cells induced a 20-fold increase in live TC-1 proliferation in vitro. Furthermore, subcutaneous co-injection of irradiated TC-1 cells with TC-1 expressing luciferase (TC-1 fluc<sup>+</sup>) markedly increased TC-1 fluc<sup>+</sup> proliferation in a receptor-dependent way. Moreover we used a human carcinoma cell line not expressing the PAF receptor (KBM) and the same cell transfected with the receptor gene (KBP). Following co-injection of live KBP cells with irradiated KBM in RAG mice, the tumor growth was significantly increased compared with tumor formed following co-injection of live KBM with irradiated KBM. This tumor cell repopulation correlated with increased infiltration of tumor-promoting macrophages (CD206+). We propose that receptor represents a possible target for improving the efficacy of radiotherapy through inhibition of tumor repopulation."],"journal":["Oncogenesis"],"pagination":["e296"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5294253"],"repository":["biostudies-literature"],"pubmed_title":["Platelet-activating factor (PAF) receptor as a promising target for cancer cell repopulation after radiotherapy."],"pmcid":["PMC5294253"],"pubmed_authors":["Lepique AP","da Silva IA","Jancar S","Chammas R"],"additional_accession":[]},"is_claimable":false,"name":"Platelet-activating factor (PAF) receptor as a promising target for cancer cell repopulation after radiotherapy.","description":"A major drawback of radiotherapy is the accelerated growth of the surviving tumor cells. Radiotherapy generates a variety of lipids that bind to the receptor for platelet-activating factor, expressed by cells in the tumor microenvironment. In the present study, using the TC-1 tumor cell line, we found that irradiation induced a twofold increase in receptor expression and generated agonists of receptor. Irradiated cells induced a 20-fold increase in live TC-1 proliferation in vitro. Furthermore, subcutaneous co-injection of irradiated TC-1 cells with TC-1 expressing luciferase (TC-1 fluc<sup>+</sup>) markedly increased TC-1 fluc<sup>+</sup> proliferation in a receptor-dependent way. Moreover we used a human carcinoma cell line not expressing the PAF receptor (KBM) and the same cell transfected with the receptor gene (KBP). Following co-injection of live KBP cells with irradiated KBM in RAG mice, the tumor growth was significantly increased compared with tumor formed following co-injection of live KBM with irradiated KBM. This tumor cell repopulation correlated with increased infiltration of tumor-promoting macrophages (CD206+). We propose that receptor represents a possible target for improving the efficacy of radiotherapy through inhibition of tumor repopulation.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jan","modification":"2025-04-22T19:19:03.736Z","creation":"2019-03-27T02:35:52Z"},"accession":"S-EPMC5294253","cross_references":{"pubmed":["28134937"],"doi":["10.1038/oncsis.2016.90"]}}