biostudies-literatureCui ZShanghai health and family planning committeepre-outstanding medical youth program of Shanghai General HospitaNational Natural Science Foundation of China (CN)Shanghai Jiao Tong University School of medicine, Doctor Innovation FundShanghai Sailing Program26https://www.ebi.ac.uk/biostudies/studies/S-EPMC5299689biostudies-literatureUnknown14(1)<h4>Background</h4>The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria.<h4>Methods</h4>Staphylococcus phage JD007 was characterized and its complete genome sequence analysed.<h4>Results</h4>Staphylococcus phage JD007 was classified as belonging to the Myoviridae family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5-11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation. The complete genome size is 141,836 bp, making JD007 one of the largest Staphylococcus phages of Myoviridae. No identifiable resistance or virulence genes were found in the JD007 genome. JD007 was ableVirology journalCharacterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China.PMC529968931500154201440289BXJ20120115YF140950006N1503012Zhu YHan LGu FDong KZhang YQin JGuo XCui ZFeng TLi QfalseCharacterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China.<h4>Background</h4>The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria.<h4>Methods</h4>Staphylococcus phage JD007 was characterized and its complete genome sequence analysed.<h4>Results</h4>Staphylococcus phage JD007 was classified as belonging to the Myoviridae family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5-11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation. The complete genome size is 141,836 bp, making JD007 one of the largest Staphylococcus phages of Myoviridae. No identifiable resistance or virulence genes were found in the JD007 genome. JD007 was able2017-01-01T00:00:00Z2017 Feb2026-06-07T03:28:52.28Z2019-03-27T02:36:10ZS-EPMC52996892817901010.1186/s12985-017-0701-0