{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Riehl BD"],"funding":["National Science Foundation CAREER","Layman New Directions Award","National Institute of General Medical Sciences"],"pagination":["e0171857"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5310897"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(2)"],"pubmed_abstract":["Despite the important role of mechanical signals in bone remodeling, relatively little is known about how fluid shear affects osteoblastic cell migration behavior. Here we demonstrated that MC3T3-E1 osteoblast migration could be activated by physiologically-relevant levels of fluid shear in a shear stress-dependent manner. Interestingly, shear-sensitive osteoblast migration behavior was prominent only during the initial period after the onset of the steady flow (for about 30 min), exhibiting shear stress-dependent migration speed, displacement, arrest coefficient, and motility coefficient. For example, cell speed at 1 min was 0.28, 0.47, 0.51, and 0.84 μm min-1 for static, 2, 15, and 25 dyne cm-2 shear stress, respectively. Arrest coefficient (measuring how often cells are paused during mi"],"journal":["PloS one"],"pubmed_title":["Flowtaxis of osteoblast migration under fluid shear and the effect of RhoA kinase silencing."],"pmcid":["PMC5310897"],"funding_grant_id":["P20GM104320","1351570"],"pubmed_authors":["Lee JS","Riehl BD","Kwon IK","Lim JY","Ha L"],"additional_accession":[]},"is_claimable":false,"name":"Flowtaxis of osteoblast migration under fluid shear and the effect of RhoA kinase silencing.","description":"Despite the important role of mechanical signals in bone remodeling, relatively little is known about how fluid shear affects osteoblastic cell migration behavior. Here we demonstrated that MC3T3-E1 osteoblast migration could be activated by physiologically-relevant levels of fluid shear in a shear stress-dependent manner. Interestingly, shear-sensitive osteoblast migration behavior was prominent only during the initial period after the onset of the steady flow (for about 30 min), exhibiting shear stress-dependent migration speed, displacement, arrest coefficient, and motility coefficient. For example, cell speed at 1 min was 0.28, 0.47, 0.51, and 0.84 μm min-1 for static, 2, 15, and 25 dyne cm-2 shear stress, respectively. Arrest coefficient (measuring how often cells are paused during mi","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2025-04-06T13:54:32.836Z","creation":"2019-03-26T22:49:13Z"},"accession":"S-EPMC5310897","cross_references":{"pubmed":["28199362"],"doi":["10.1371/journal.pone.0171857"]}}