biostudies-literatureRiehl BDNational Science Foundation CAREERLayman New Directions AwardNational Institute of General Medical Sciencese0171857https://www.ebi.ac.uk/biostudies/studies/S-EPMC5310897biostudies-literatureUnknown12(2)Despite the important role of mechanical signals in bone remodeling, relatively little is known about how fluid shear affects osteoblastic cell migration behavior. Here we demonstrated that MC3T3-E1 osteoblast migration could be activated by physiologically-relevant levels of fluid shear in a shear stress-dependent manner. Interestingly, shear-sensitive osteoblast migration behavior was prominent only during the initial period after the onset of the steady flow (for about 30 min), exhibiting shear stress-dependent migration speed, displacement, arrest coefficient, and motility coefficient. For example, cell speed at 1 min was 0.28, 0.47, 0.51, and 0.84 μm min-1 for static, 2, 15, and 25 dyne cm-2 shear stress, respectively. Arrest coefficient (measuring how often cells are paused during miPloS oneFlowtaxis of osteoblast migration under fluid shear and the effect of RhoA kinase silencing.PMC5310897P20GM1043201351570Lee JSRiehl BDKwon IKLim JYHa LfalseFlowtaxis of osteoblast migration under fluid shear and the effect of RhoA kinase silencing.Despite the important role of mechanical signals in bone remodeling, relatively little is known about how fluid shear affects osteoblastic cell migration behavior. Here we demonstrated that MC3T3-E1 osteoblast migration could be activated by physiologically-relevant levels of fluid shear in a shear stress-dependent manner. Interestingly, shear-sensitive osteoblast migration behavior was prominent only during the initial period after the onset of the steady flow (for about 30 min), exhibiting shear stress-dependent migration speed, displacement, arrest coefficient, and motility coefficient. For example, cell speed at 1 min was 0.28, 0.47, 0.51, and 0.84 μm min-1 for static, 2, 15, and 25 dyne cm-2 shear stress, respectively. Arrest coefficient (measuring how often cells are paused during mi2017-01-01T00:00:00Z20172025-04-06T13:54:32.836Z2019-03-26T22:49:13ZS-EPMC53108972819936210.1371/journal.pone.0171857