biostudies-literatureUnknown7Gimenez MJSome studies have suggested that the immunogenicity of oats depends on the cultivar. RP-HPLC has been proposed as a useful technique to select varieties of oats with reduced immunogenicity. The aim of this study was to identify both the avenin protein patterns associated with low gluten content and the available variability for the development of new non-toxic oat cultivars. The peaks of alcohol-soluble avenins of a collection of landraces and cultivars of oats have been characterized based on the RP-HPLC elution times. The immunotoxicity of oat varieties for patients with celiac disease (CD) has been tested using a competitive ELISA based on G12 monoclonal antibody. The oat lines show, on average, seven avenin peaks giving profiles with certain similarities. Based on this similarity, mostScientific reports42588https://www.ebi.ac.uk/biostudies/studies/S-EPMC5314403biostudies-literatureCharacterization of celiac disease related oat proteins: bases for the development of high quality oat varieties suitable for celiac patients.PMC5314403Gimenez MJReal ABarro FGarcia-Molina MDSousa CfalseCharacterization of celiac disease related oat proteins: bases for the development of high quality oat varieties suitable for celiac patients.Some studies have suggested that the immunogenicity of oats depends on the cultivar. RP-HPLC has been proposed as a useful technique to select varieties of oats with reduced immunogenicity. The aim of this study was to identify both the avenin protein patterns associated with low gluten content and the available variability for the development of new non-toxic oat cultivars. The peaks of alcohol-soluble avenins of a collection of landraces and cultivars of oats have been characterized based on the RP-HPLC elution times. The immunotoxicity of oat varieties for patients with celiac disease (CD) has been tested using a competitive ELISA based on G12 monoclonal antibody. The oat lines show, on average, seven avenin peaks giving profiles with certain similarities. Based on this similarity, most2017-01-01T00:00:00Z2017 Feb2025-05-29T19:55:37.998Z2025-05-29T19:55:37.998ZS-EPMC53144032820996210.1038/srep42588