{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Spaethling JM"],"funding":["NIMH NIH HHS","NIH Single Cell Analysis Program"],"pagination":["791-803"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5316103"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(3)"],"pubmed_abstract":["Investigation of human CNS disease and drug effects has been hampered by the lack of a system that enables single-cell analysis of live adult patient brain cells. We developed a culturing system, based on a papain-aided procedure, for resected adult human brain tissue removed during neurosurgery. We performed single-cell transcriptomics on over 300 cells, permitting identification of oligodendrocytes, microglia, neurons, endothelial cells, and astrocytes after 3 weeks in culture. Using deep sequencing, we detected over 12,000 expressed genes, including hundreds of cell-type-enriched mRNAs, lncRNAs and pri-miRNAs. We describe cell-type- and patient-specific transcriptional hierarchies. Single-cell transcriptomics on cultured live adult patient derived cells is a prime example of the promise"],"journal":["Cell reports"],"pubmed_title":["Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics."],"pmcid":["PMC5316103"],"funding_grant_id":["R33 MH106637","U01 MH098953","MH106637"],"pubmed_authors":["Sul JY","Grady MS","Spaethling JM","Lucas TH","Lee J","Chen HI","Dueck H","Na YJ","Bell TJ","Khaladkar M","Kim J","Wang J","Wolf JA","Fisher SA","Ulyanova AV","Kung DK","Baltuch GH","Garcia MP","O'Rourke DM","Bartfai T","Stefanik D","Eberwine JH","Brem S"],"additional_accession":[]},"is_claimable":false,"name":"Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics.","description":"Investigation of human CNS disease and drug effects has been hampered by the lack of a system that enables single-cell analysis of live adult patient brain cells. We developed a culturing system, based on a papain-aided procedure, for resected adult human brain tissue removed during neurosurgery. We performed single-cell transcriptomics on over 300 cells, permitting identification of oligodendrocytes, microglia, neurons, endothelial cells, and astrocytes after 3 weeks in culture. Using deep sequencing, we detected over 12,000 expressed genes, including hundreds of cell-type-enriched mRNAs, lncRNAs and pri-miRNAs. We describe cell-type- and patient-specific transcriptional hierarchies. Single-cell transcriptomics on cultured live adult patient derived cells is a prime example of the promise","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Jan","modification":"2026-04-14T09:35:56.922Z","creation":"2019-03-27T02:36:50Z"},"accession":"S-EPMC5316103","cross_references":{"pubmed":["28099855"],"doi":["10.1016/j.celrep.2016.12.066"]}}