{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["van Munster JM"],"funding":["Biotechnology and Biological Sciences Research Council"],"pagination":["43117"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5318901"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7"],"pubmed_abstract":["Renewables-based biotechnology depends on enzymes to degrade plant lignocellulose to simple sugars that are converted to fuels or high-value products. Identification and characterization of such lignocellulose degradative enzymes could be fast-tracked by availability of an enzyme activity measurement method that is fast, label-free, uses minimal resources and allows direct identification of generated products. We developed such a method by applying carbohydrate arrays coupled with MALDI-ToF mass spectrometry to identify reaction products of carbohydrate active enzymes (CAZymes) of the filamentous fungus Aspergillus niger. We describe the production and characterization of plant polysaccharide-derived oligosaccharides and their attachment to hydrophobic self-assembling monolayers on a gold "],"journal":["Scientific reports"],"pubmed_title":["Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger."],"pmcid":["PMC5318901"],"funding_grant_id":["BB/M028836/1","BB/K01434X/1","BB/G01616X/1","BB/M029034/1"],"pubmed_authors":["Flitsch SL","van Munster JM","Archer DB","Thomas B","Gray CJ","Davis AL","Riese M"],"additional_accession":[]},"is_claimable":false,"name":"Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger.","description":"Renewables-based biotechnology depends on enzymes to degrade plant lignocellulose to simple sugars that are converted to fuels or high-value products. Identification and characterization of such lignocellulose degradative enzymes could be fast-tracked by availability of an enzyme activity measurement method that is fast, label-free, uses minimal resources and allows direct identification of generated products. We developed such a method by applying carbohydrate arrays coupled with MALDI-ToF mass spectrometry to identify reaction products of carbohydrate active enzymes (CAZymes) of the filamentous fungus Aspergillus niger. We describe the production and characterization of plant polysaccharide-derived oligosaccharides and their attachment to hydrophobic self-assembling monolayers on a gold ","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Feb","modification":"2026-05-30T14:26:35.932Z","creation":"2019-03-27T02:37:00Z"},"accession":"S-EPMC5318901","cross_references":{"pubmed":["28220903"],"doi":["10.1038/srep43117"]}}