{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Choi SY"],"funding":["Basic Science Research Program through National Research Foundation of Korea"],"pagination":["14"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5319158"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(1)"],"pubmed_abstract":["<h4>Background</h4>Side-population (SP) cells that exclude anti-cancer drugs have been found in various tumor cell lines. Moreover, SP cells have a higher proliferative potential and drug resistance than main population cells (Non-SP cells). Also, several ion channels are responsible for the drug resistance and proliferation of SP cells in cancer.<h4>Methods</h4>To confirm the expression and function of voltage-gated potassium (Kv) channels of SP cells, these cells, as well as highly expressed ATP-binding cassette (ABC) transporters and stemness genes, were isolated from a gefitinib-resistant human lung adenocarcinoma cell line (NCI-H460), using Hoechst 33342 efflux.<h4>Results</h4>In the present study, we found that mRNA expression of Kv channels in SP cells was different compared to Non-SP cells, and the resistance of SP cells to gefitinib was weakened with a combination treatment of gefitinib and Kv channel blockers or a Kv7 opener, compared to single-treatment gefitinib, through inhibition of the Ras-Raf signaling pathway.<h4>Conclusions</h4>The findings indicate that Kv channels in SP cells could be new targets for reducing the resistance to gefitinib."],"journal":["BMC pharmacology & toxicology"],"pubmed_title":["Regulation of voltage-gated potassium channels attenuates resistance of side-population cells to gefitinib in the human lung cancer cell line NCI-H460."],"pmcid":["PMC5319158"],"funding_grant_id":["NRF-2014R1A1A3A04052757"],"pubmed_authors":["Choi SY","Lee SY","Ryu PD","Kim HR"],"additional_accession":[]},"is_claimable":false,"name":"Regulation of voltage-gated potassium channels attenuates resistance of side-population cells to gefitinib in the human lung cancer cell line NCI-H460.","description":"<h4>Background</h4>Side-population (SP) cells that exclude anti-cancer drugs have been found in various tumor cell lines. Moreover, SP cells have a higher proliferative potential and drug resistance than main population cells (Non-SP cells). Also, several ion channels are responsible for the drug resistance and proliferation of SP cells in cancer.<h4>Methods</h4>To confirm the expression and function of voltage-gated potassium (Kv) channels of SP cells, these cells, as well as highly expressed ATP-binding cassette (ABC) transporters and stemness genes, were isolated from a gefitinib-resistant human lung adenocarcinoma cell line (NCI-H460), using Hoechst 33342 efflux.<h4>Results</h4>In the present study, we found that mRNA expression of Kv channels in SP cells was different compared to Non-SP cells, and the resistance of SP cells to gefitinib was weakened with a combination treatment of gefitinib and Kv channel blockers or a Kv7 opener, compared to single-treatment gefitinib, through inhibition of the Ras-Raf signaling pathway.<h4>Conclusions</h4>The findings indicate that Kv channels in SP cells could be new targets for reducing the resistance to gefitinib.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Feb","modification":"2024-12-04T02:23:00.56Z","creation":"2019-06-06T17:02:58Z"},"accession":"S-EPMC5319158","cross_references":{"pubmed":["28219421"],"doi":["10.1186/s40360-017-0118-9"]}}