{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8"],"submitter":["Han S"],"pubmed_abstract":["Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including N<sup>ɛ</sup>-acetyl-lysine. Stable integration of transgenes encoding an engineered N<sup>ɛ</sup>-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNA<sup>Pyl</sup> pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease."],"journal":["Nature communications"],"pagination":["14568"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5321798"],"repository":["biostudies-literature"],"pubmed_title":["Expanding the genetic code of Mus musculus."],"pmcid":["PMC5321798"],"pubmed_authors":["Lee S","Park HS","Park CB","Yang A","Han S","Lee HW"],"additional_accession":[]},"is_claimable":false,"name":"Expanding the genetic code of Mus musculus.","description":"Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including N<sup>ɛ</sup>-acetyl-lysine. Stable integration of transgenes encoding an engineered N<sup>ɛ</sup>-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNA<sup>Pyl</sup> pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Feb","modification":"2025-04-18T19:22:54.003Z","creation":"2019-03-27T02:37:09Z"},"accession":"S-EPMC5321798","cross_references":{"pubmed":["28220771"],"doi":["10.1038/ncomms14568"]}}