{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Koh B"],"funding":["NIAID NIH HHS"],"pagination":["2165-2171"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5321815"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["198(5)"],"pubmed_abstract":["IL-10 is an immunoregulatory cytokine that has broad effects across the immune system. In Th cell subsets, Th2 cells produce considerable amounts of IL-10. The transcription factors that regulate IL-10 production in Th2 cells are still incompletely described. In this study, we demonstrate that the ETS family transcription factor ETS variant (Etv)5 regulates IL-10 production in Th2 cells. T cell-specific Etv5-deficient and littermate control mice demonstrated that IL-10 production and gene expression were significantly decreased in the absence of Etv5. In an <i>Aspergillus fumigatus</i> extract-induced inflammation model, IL-10-producing CD4<sup>+</sup> T cells in bronchoalveolar lavage and lung were significantly decreased in mice that lacked Etv5 in T cells, compared with control mice. We showed that Etv5 directly binds to the <i>Il10</i> locus conserved noncoding sequence 3 site and that it activates gene expression in a luciferase reporter assay and following retroviral transduction. Etv5 deficiency did not affect the expression of other transcription factors known to be important for expression of IL-10, including Jun family members, GATA3, E4BP4, and IFN regulatory factor 4. However, in the absence of Etv5, binding of these transcription factors to the <i>Il10</i> locus was dramatically reduced. Ectopic Etv5 expression in Th2 cells that lack Etv5 restored IL-10 production and the binding of IL-10-inducing transcription factors including E4BP4, IFN regulatory factor 4, and GATA3. Taken together, we conclude that Etv5 plays a crucial role in regulating IL-10 production in Th2 cells by facilitating the binding of IL-10-inducing transcription factors at the <i>Il10</i> locus."],"journal":["Journal of immunology (Baltimore, Md. : 1950)"],"pubmed_title":["Etv5 Regulates IL-10 Production in Th Cells."],"pmcid":["PMC5321815"],"funding_grant_id":["R56 AI045515","T32 AI060519","R01 AI045515","R01 AI057459"],"pubmed_authors":["Kaplan MH","Koh B","Hufford MM","Sun X"],"additional_accession":[]},"is_claimable":false,"name":"Etv5 Regulates IL-10 Production in Th Cells.","description":"IL-10 is an immunoregulatory cytokine that has broad effects across the immune system. In Th cell subsets, Th2 cells produce considerable amounts of IL-10. The transcription factors that regulate IL-10 production in Th2 cells are still incompletely described. In this study, we demonstrate that the ETS family transcription factor ETS variant (Etv)5 regulates IL-10 production in Th2 cells. T cell-specific Etv5-deficient and littermate control mice demonstrated that IL-10 production and gene expression were significantly decreased in the absence of Etv5. In an <i>Aspergillus fumigatus</i> extract-induced inflammation model, IL-10-producing CD4<sup>+</sup> T cells in bronchoalveolar lavage and lung were significantly decreased in mice that lacked Etv5 in T cells, compared with control mice. We showed that Etv5 directly binds to the <i>Il10</i> locus conserved noncoding sequence 3 site and that it activates gene expression in a luciferase reporter assay and following retroviral transduction. Etv5 deficiency did not affect the expression of other transcription factors known to be important for expression of IL-10, including Jun family members, GATA3, E4BP4, and IFN regulatory factor 4. However, in the absence of Etv5, binding of these transcription factors to the <i>Il10</i> locus was dramatically reduced. Ectopic Etv5 expression in Th2 cells that lack Etv5 restored IL-10 production and the binding of IL-10-inducing transcription factors including E4BP4, IFN regulatory factor 4, and GATA3. Taken together, we conclude that Etv5 plays a crucial role in regulating IL-10 production in Th2 cells by facilitating the binding of IL-10-inducing transcription factors at the <i>Il10</i> locus.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Mar","modification":"2025-04-04T02:08:38.781Z","creation":"2019-03-26T23:04:08Z"},"accession":"S-EPMC5321815","cross_references":{"pubmed":["28100679"],"doi":["10.4049/jimmunol.1600801"]}}