{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Songsasen N"],"funding":["Research Infrastructure Programs/Office of the Director","National Center for Research Resources","NCRR NIH HHS","National Institutes of Health,","NIH HHS"],"pagination":["228-236"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5325155"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["90"],"pubmed_abstract":["Using the domestic cat as a non-rodent, larger animal model, the objective was to determine the impact of a brief incubation in a hypertonic microenvironment on (1) ovarian follicle and oocyte growth in vitro, (2) developmental capacity of the resident oocyte, and (3) expression of aquaporin (AQP) genes in parallel with genes involved in regulation of folliculogenesis. In Study 1: Secondary or early antral follicles encapsulated in 0.5% alginate were allocated to one of three treatment groups: 1) culture in standard medium at 290 mOsm for 15 d (Control); 2) incubation in 350 mOsm medium for 1 h followed by culture in standard medium for 15 d (Hypertonic-1h); or 3) incubation in 350 mOsm medium for 24 h followed by incubation in standard medium for additional 14 d (Hypertonic-24h). After me"],"journal":["Theriogenology"],"pubmed_title":["Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model."],"pmcid":["PMC5325155"],"funding_grant_id":["R01 RR026064","R01 R026064","R01 OD010948","R01 OD 010948"],"pubmed_authors":["Wildt DE","Songsasen N","Comizzoli P","Thongkittidilok C","Yamamizu K"],"additional_accession":[]},"is_claimable":false,"name":"Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model.","description":"Using the domestic cat as a non-rodent, larger animal model, the objective was to determine the impact of a brief incubation in a hypertonic microenvironment on (1) ovarian follicle and oocyte growth in vitro, (2) developmental capacity of the resident oocyte, and (3) expression of aquaporin (AQP) genes in parallel with genes involved in regulation of folliculogenesis. In Study 1: Secondary or early antral follicles encapsulated in 0.5% alginate were allocated to one of three treatment groups: 1) culture in standard medium at 290 mOsm for 15 d (Control); 2) incubation in 350 mOsm medium for 1 h followed by culture in standard medium for 15 d (Hypertonic-1h); or 3) incubation in 350 mOsm medium for 24 h followed by incubation in standard medium for additional 14 d (Hypertonic-24h). After me","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Mar","modification":"2025-04-21T18:50:05.135Z","creation":"2020-11-07T09:52:09Z"},"accession":"S-EPMC5325155","cross_references":{"pubmed":["28166973"],"doi":["10.1016/j.theriogenology.2016.12.006"]}}