{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":60,"searchCount":0},"additional":{"submitter":["Ramani V"],"funding":["NIDDK NIH HHS","Howard Hughes Medical Institute","NHGRI NIH HHS"],"pagination":["263-266"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5330809"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(3)"],"pubmed_abstract":["We present single-cell combinatorial indexed Hi-C (sciHi-C), a method that applies combinatorial cellular indexing to chromosome conformation capture. In this proof of concept, we generate and sequence six sciHi-C libraries comprising a total of 10,696 single cells. We use sciHi-C data to separate cells by karyotypic and cell-cycle state differences and identify cell-to-cell heterogeneity in mammalian chromosomal conformation. Our results demonstrate that combinatorial indexing is a generalizable strategy for single-cell genomics."],"journal":["Nature methods"],"pubmed_title":["Massively multiplex single-cell Hi-C."],"pmcid":["PMC5330809"],"funding_grant_id":["DP1 HG007811","R01 HG006283","U54 DK107979","T32 HG000035"],"pubmed_authors":["Duan Z","Ramani V","Steemers FJ","Shendure J","Noble WS","Qiu R","Disteche CM","Gunderson KL","Deng X"],"view_count":["60"],"additional_accession":[]},"is_claimable":false,"name":"Massively multiplex single-cell Hi-C.","description":"We present single-cell combinatorial indexed Hi-C (sciHi-C), a method that applies combinatorial cellular indexing to chromosome conformation capture. In this proof of concept, we generate and sequence six sciHi-C libraries comprising a total of 10,696 single cells. We use sciHi-C data to separate cells by karyotypic and cell-cycle state differences and identify cell-to-cell heterogeneity in mammalian chromosomal conformation. Our results demonstrate that combinatorial indexing is a generalizable strategy for single-cell genomics.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Mar","modification":"2024-10-15T04:56:59.919Z","creation":"2019-03-27T02:37:33Z"},"accession":"S-EPMC5330809","cross_references":{"pubmed":["28135255"],"doi":["10.1038/nmeth.4155"]}}