{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bin Mohamed Suffian IF"],"funding":["Biotechnology and Biological Sciences Research Council"],"pagination":["43160"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5335696"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7"],"pubmed_abstract":["An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E. coli is required to improve laboratory-scale productivity for further drug delivery applications. Production steps involve bacterial culture, protein isolation, denaturation, purification and finally protein assembly. In this study, we describe a modified E. coli based method for purifying HBc particles and compare the results with those obtained using a conventional purification method. HBc particle morphology was confirmed by Atomic Force Microscopy (AFM). Protein specificity and secondary structure were confirmed by Western Blot and Circ"],"journal":["Scientific reports"],"pubmed_title":["Yield Optimisation of Hepatitis B Virus Core Particles in E. coli Expression System for Drug Delivery Applications."],"pmcid":["PMC5335696"],"funding_grant_id":["BB/J008656/1"],"pubmed_authors":["Brown P","Bui T","Nishimura Y","Garcia-Maya M","Palermo AR","Bin Mohamed Suffian IF","Al-Jamal KT","Kondo A","Ogino C"],"additional_accession":[]},"is_claimable":false,"name":"Yield Optimisation of Hepatitis B Virus Core Particles in E. coli Expression System for Drug Delivery Applications.","description":"An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E. coli is required to improve laboratory-scale productivity for further drug delivery applications. Production steps involve bacterial culture, protein isolation, denaturation, purification and finally protein assembly. In this study, we describe a modified E. coli based method for purifying HBc particles and compare the results with those obtained using a conventional purification method. HBc particle morphology was confirmed by Atomic Force Microscopy (AFM). Protein specificity and secondary structure were confirmed by Western Blot and Circ","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Mar","modification":"2026-05-30T04:21:34.351Z","creation":"2019-03-27T02:37:46Z"},"accession":"S-EPMC5335696","cross_references":{"pubmed":["28256592"],"doi":["10.1038/srep43160"]}}